Fecal microbiota transplantation from young donor mice improves ovarian function in aged mice
Fecal microbiota transplantation from young donor mice improves ovarian function in aged mice
复制标题
年轻供体小鼠的粪便微生物群移植可改善老年小鼠的卵巢功能
DOI：
10.1016/j.jgg.2022.05.006
复制
发表时间：
2022
影响因子：
5.9
通讯作者：
Jing Li
中科院分区：
文献类型：
--
作者：
Li Xu;Qiankun Zhang;Xiaowei Dou;Yipeng Wang;Jianwei Wang;Yong Zhou;Xingyin Liu;Jing Li
高龄产妇的特点是卵巢中卵母细胞的数量和质量下降，衰老过程伴随着肠道微生物群组成的变化。然而，人们对肠道微生物群与卵巢衰老之间的关系知之甚少。通过使用粪便微生物群移植（FMT）将材料从年轻（5周龄）移植到老年（42周龄）小鼠中，我们发现FMT处理的小鼠中的肠道微生物群的组成呈现出“类双歧杆菌表型”和肠道细菌（如双歧杆菌和瘤胃球菌科）的增加。此外，FMT处理的小鼠显示抗炎细胞因子IL-4增加和促炎细胞因子IFN-γ减少。用于评估卵巢功能的生育力测试显示，FMT处理的雌性小鼠的第一窝仔数显著高于非FMT组。形态学分析表明，FMT处理小鼠卵巢中卵泡闭锁和凋亡的显著减少以及细胞增殖的增加。我们的研究结果还表明，FMT改善了衰老卵巢的免疫微环境，减少了巨噬细胞和巨噬细胞衍生的多核巨细胞（MNGC）。这些结果表明，来自年轻供体的FMT可能是延缓卵巢衰老的良好选择。
Advanced maternal age is characterized by declines in the quantity and quality of oocytes in the ovaries, and the aging process is accompanied by changes in gut microbiota composition. However, little is known about the relationship between gut microbiota and ovarian aging. By using fecal microbiota transplantation (FMT) to transplant material from young (5-week-old) into aged (42-week-old) mice, we find that the composition of gut microbiota in FMT-treated mice presents a “younger-like phenotype” and an increase of commensal bacteria, such as Bifidobacterium and Ruminococcaceae . Moreover, the FMT-treated mice show increased anti-inflammatory cytokine IL-4 and decreased pro-inflammatory cytokine IFN-γ. Fertility tests for assessing ovarian function reveal that the first litter size of female FMT-treated mice is significantly higher than that of the non-FMT group. Morphology analysis demonstrates a dramatic decrease in follicle atresia and apoptosis as well as an increase in cellular proliferation in the ovaries of the FMT-treated mice. Our results also show that FMT improves the immune microenvironment in aged ovaries, with decreased macrophages and macrophage-derived multinucleated giant cells (MNGCs). These results suggest that FMT from young donors could be a good choice for delaying ovarian aging.