Effects of early endotoxemia and dextran-induced anaphylaxis on the size selectivity of the glomerular filtration barrier in rats
Effects of early endotoxemia and dextran-induced anaphylaxis on the size selectivity of the glomerular filtration barrier in rats
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DOI：
10.1152/ajprenal.90263.2008
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发表时间：
2009-02-01
影响因子：
4.2
通讯作者：
Rippe, Bengt
中科院分区：
文献类型：
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作者：
Axelsson, Josefin;Rippe, Anna;Rippe, Bengt
Axelsson J、Rippe A、Venturoli D、Sward P、Rippe B。早期内毒素血症和右旋糖酐诱导的过敏反应对大鼠肾小球滤过屏障大小选择性的影响。 Am J Physiol Renal Physiol 296：F242-F248，2009。首次发表于 2008 年 11 月 12 日； doi:10.1152/ajprenal.90263.2008.-本研究旨在调查导致内毒素血症和过敏性休克期间发生微量白蛋白尿的肾小球通透性变化。在麻醉的 Wistar 大鼠中，将导管插入左侧输尿管以收集尿液，同时实现血液通路。内毒素血症由大肠杆菌脂多糖 (LPS) 诱导，并在 60、90 (n = 7) 和 120 (n = 7) 分钟时评估肾小球通透性。通过推注剂量的右旋糖酐-70诱导过敏反应，并在 5 分钟 (n = 8) 和 40 分钟 (n = 9) 时评估肾小球通透性。假手术动物被跟踪 5 或 120 分钟。从血浆和尿液样本中测定异硫氰酸荧光素-Ficoll (70/400) 的肾小球筛分系数 (theta)，并使用尺寸排阻色谱 (HPLC) 进行评估。开始LPS输注后（2小时），但不是在60或90分钟时，Ficoll的theta（70埃）显着增加[从2.91 x 10(-5) +/- 6.33 x 10(-6)到7.78 x 10(-5) +/- 6.21 x 10(-6) (P < 0.001)]。在过敏反应中，分子半径 > 60 埃的聚蔗糖的 θ 在 5 分钟时已大幅增加，但肾小球通透性在 40 分钟时完全恢复。总之，在右旋糖酐引起的过敏反应中，肾小球通透性出现短暂、立即的增加，并且在 40 分钟内完全可逆。相比之下，内毒素血症导致肾小球通透性增加，这在 2 小时后首先显现出来。在这两种情况下，大聚蔗糖分子的 theta 值均显着增加，反映出肾小球过滤器中大孔数量的增加。
Axelsson J, Rippe A, Venturoli D, Sward P, Rippe B. Effects of early endotoxemia and dextran-induced anaphylaxis on the size selectivity of the glomerular filtration barrier in rats. Am J Physiol Renal Physiol 296: F242-F248, 2009. First published November 12, 2008; doi:10.1152/ajprenal.90263.2008.-This study was performed to investigate the glomerular permeability alterations responsible for the microalbuminuria occurring in endotoxemia and during anaphylactic shock. In anesthetized Wistar rats, the left ureter was catheterized for urine collection while, simultaneously, blood access was achieved. Endotoxemia was induced by lipopolysaccharide (LPS) from Escherichia coli, and glomerular permeability was assessed at 60 and 90 (n = 7) and 120 (n = 7) min. Anaphylaxis was induced by a bolus dose of Dextran-70, and glomerular permeability assessed at 5 min (n = 8) and 40 min (n = 9). Sham animals were followed for either 5 or 120 min. The glomerular sieving coefficients (theta) to fluorescein isothiocyanate-Ficoll (70/400) were determined from plasma and urine samples and assessed using size-exclusion chromatography (HPLC). After start of the LPS infusion (2 h), but not at 60 or 90 min, theta for Ficoll(70 angstrom) had increased markedly [from 2.91 x 10(-5) +/- 6.33 x 10(-6) to 7.78 x 10(-5) +/- 6.21 x 10(-6) (P 0.001)]. In anaphylaxis, there was a large increase in theta for Ficolls > 60 angstrom in molecular radius already at 5 min, but the glomerular permeability was completely restored at 40 min. In conclusion, there was a transient, immediate increment of glomerular permeability in dextran-induced anaphylaxis, which was completely reversible within 40 min. By contrast, endotoxemia caused an increase in glomerular permeability that was manifest first after 2 h. In both cases, theta to large Ficoll molecules were markedly increased, reflecting an increase in the number of large pores in the glomerular filter.