HEPATOCYTE CELL-SURFACE POLARITY AS DEMONSTRATED BY LECTIN BINDING
HEPATOCYTE CELL-SURFACE POLARITY AS DEMONSTRATED BY LECTIN BINDING
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DOI：
10.1177/36.12.2848070
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发表时间：
1988-12-01
影响因子：
3.2
通讯作者：
JAUREGUI, HO
中科院分区：
文献类型：
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作者：
MCMILLAN, PN;HIXSON, DC;JAUREGUI, HO
我们进行了调查，在超微结构水平的差异性分布之间的肝窦，侧，和胆管域的成年大鼠肝细胞凝集素结合位点。凝集素结合肝细胞glycocalices进行了研究，在原位或细胞解离后，通过酶（胶原酶），化学（EDTA），和机械方法，以及在细胞培养过程中。使用13个生物素化凝集素和抗生物素蛋白-生物素-过氧化物酶复合物（ABC），我们已经确定了凝集素结合位点，主要定位于胆小管[蓖麻凝集素（RCA）]或窦[菜豆（PHA）]域原位和机械解离的细胞。小透镜（LCA）染色在窦状隙表面显著，沿着侧面轻微，在胆小管区域完全不存在。伴刀豆球蛋白A（ConA）是唯一的结合平等的所有结构域。小麦[小麦胚芽凝集素（WGA）]也结合到所有域，但最强烈的胆小管区域。通过胶原酶或EDTA处理解离的细胞表现出球形形态，其特征在于许多表面微绒毛和缺乏形态结构域。凝集素结合解离的细胞均匀分布在整个细胞表面，这表明凝集素受体的重新分配是独立的分离程序。培养的肝细胞表现出部分恢复的形态结构域，但凝集素结合极性没有重新建立。
We performed an investigation at the ultrastructural level of the differential distribution of lectin-binding sites among sinusoidal, lateral, and bile canailcular domains of adult rat hepatocytes. Lectin binding to hepatocyt glycocalices was studied in situ or after cellular dissociation by enzymatic (collagenase), chemical (EDTA), and mechanical methods, as well as during cell culture. Using thirteen biotinylated lectins and an avidin-biotin-peroxidase complex (ABC), we have identified lectin-binding sites that are predominantly localized in the bile canalicular [Ricinus communis agglutinin (RCA)] or sinusoidal [Phaseolus vulgaris (PHA)] domains in situ and in mechanically dissociated cells. Lens culinaris (LCA) staining was prominent on sinusoidal surfaces, slight along lateral surfaces, and completely absent in the bile canalicular domain. Concanavalin A (Con A) was unique in binding equally to all domains. Triticum vulgare [wheat germ agglutinin (WGA)] was also bound to all domains, but most intensely to the bile canalicular region. Cells dissociated via collagenase or EDTA treatment exhibited a spherical morphology characterized by many surface microvilli and absence of morphological domains. Lectin binding to dissociated cells was uniformly distributed over the entire cell surface, suggesting a redistribution of lectin receptors that was independent of the separation procedure. Hepatocytes in culture exhibited a partial restoration of morphological domains, but lectin binding polarity was not re-established.