Yeast phospholipase D, Spo14, is not required for macroautophagy
Yeast phospholipase D, Spo14, is not required for macroautophagy
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巨自噬不需要酵母磷脂酶 D、Spo14
DOI：
10.1002/yea.3803
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发表时间：
2022-06-27
期刊：
影响因子：
2.6
通讯作者：
Liang, Yongheng
中科院分区：
文献类型：
--
作者：
Chen, Yun;Wu, Zulin;Liang, Yongheng
自噬相关基因（Atg）蛋白是自噬的关键参与者。一些在囊泡运输和脂质代谢中起作用的蛋白质也参与自噬。酵母中编码磷脂酶D（PLD）的SPO 14参与细胞膜运输，在减数分裂过程中的孢子形成中起重要作用。自噬和孢子形成之间的串扰已被确定。虽然PLD是哺乳动物巨自噬所必需的，但其在酵母巨自噬中的作用仍不清楚。我们观察到Spo 14不是酵母中宏自噬所需的，并且它对于Atg 8脂化是不可或缺的，而Atg 8脂化在吞噬细胞延伸中发挥着重要作用。我们的研究结果还表明，绿色荧光蛋白（GFP）-Atg 8降解不完全阻断atg 1 Delta/atg 1 Delta细胞在孢子形成条件下。因此，Spo 14不是酵母中的宏自噬所必需的。
Autophagy-related gene (Atg) proteins are key players in autophagy. Some proteins that function in vesicle trafficking and lipid metabolism are also involved in autophagy. The SPO14 in yeast, which encodes phospholipase D (PLD), is involved in membrane trafficking and plays a vital role in sporulation during meiosis. Crosstalk has been identified between autophagy and sporulation. Although the PLD is required for macroautophagy in mammals, its role in yeast macroautophagy remains unclear. We observed that Spo14 is not required for macroautophagy in yeast and that it is dispensable for Atg8 lipidation, which plays an important role in phagophore extension. Our results also revealed that green fluorescent protein (GFP)-Atg8 degradation is not completely blocked in atg1 Delta/atg1 Delta cells under sporulation condition. Therefore, Spo14 is not required for macroautophagy in yeast.