Lactic acid bacteria display on the cell surface cytosolic proteins that recognize yeast mannan
Lactic acid bacteria display on the cell surface cytosolic proteins that recognize yeast mannan
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DOI：
10.1007/s00253-009-2295-y
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发表时间：
2010-03-01
影响因子：
5
通讯作者：
Shioya, Suteaki
中科院分区：
文献类型：
--
作者：
Katakura, Yoshio;Sano, Ryosuke;Shioya, Suteaki
蔗糖标记的转化酶，高糖基化的甘露糖蛋白从酿酒酵母，被发现结合乳酸乳球菌IL 1403在酸性pH值。使用转化酶作为配体亲和纯化的细菌的细胞壁上的蛋白质被确定为热休克蛋白，如DnaK和GroEL和糖酵解酶，如丙酮酸激酶和甘油醛-3-磷酸脱氢酶。DnaK在pH 4时与细菌和酵母结合，并在大于0.1mg/ml时使它们聚集，而在pH 4和7时DnaK的圆二色光谱之间没有观察到显著差异。这些结果表明，细胞壁上展示的包括DnaK在内的胞质蛋白导致乳酸菌粘附到酵母上。
Fluorescent-labeled invertase, a hyperglycosylated mannoprotein from Saccharomyces cerevisiae, was found to bind to Lactococcus lactis IL1403 at acidic pH. Proteins on the cell wall of the bacterium affinity-purified using invertase as a ligand were identified to be heat shock proteins such as DnaK and GroEL and glycolytic enzymes such as pyruvate kinase and glyceraldehyde-3-phosphate dehydrogenase. DnaK bound to both the bacterium and yeast at pH 4 and aggregated them at above 0.1 mg/ml, whereas no significant difference between the circular dichroism spectra of DnaK at pH 4 and 7 was observed. These results indicate that the cytosolic proteins, including DnaK displayed on the cell wall, cause the lactic acid bacterium to adhere to the yeast.