MicroRNA-128b suppresses tumor growth and promotes apoptosis by targeting A2bR in gastric cancer
MicroRNA-128b suppresses tumor growth and promotes apoptosis by targeting A2bR in gastric cancer
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MicroRNA-128b 通过靶向胃癌中的 A2bR 抑制肿瘤生长并促进细胞凋亡。
DOI：
10.1016/j.bbrc.2015.10.062
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发表时间：
2015-11-27
影响因子：
3.1
通讯作者：
He, Shuixiang
中科院分区：
文献类型：
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作者：
Wang, Ping;Guo, Xueyan;He, Shuixiang
微小RNA（microRNAs，miRNAs）在包括胃癌（gastric cancer，GC）在内的多种肿瘤的发生、发展过程中起着重要作用。miRNAs的发现为胃癌的发病机制、诊断和治疗提供了新的有力工具。本研究旨在探讨miR-128 b在胃癌发生发展中的作用及其机制。采用实时定量PCR（qRT-PCR）检测胃癌组织和胃癌细胞系中miR-128 b的表达水平。我们发现miR-128 b在胃癌组织和细胞系中显著下调。此外，miR-128 b过表达可抑制胃癌细胞的增殖、迁移和体外侵袭。体外功能获得实验进一步显示miR-128 b模拟物显著促进GC细胞凋亡。随后的双荧光素酶报告基因分析鉴定了原癌基因A2 bR之一作为miR-128 b的直接靶点。因此，我们的研究结果表明，miR-128 b是一种原癌基因miRNA，可以通过下调癌基因A2 bR来抑制GC增殖和迁移。综上所述，我们的研究结果表明，miR-128 b可以作为一个潜在的诊断生物标志物和治疗选择人类胃癌在不久的将来。(C)2015 Elsevier Inc. All rights reserved.
MicroRNAs (miRNAs) play crucial roles in the development and progression of human cancers, including gastric cancer (GC). The discovery of miRNAs may provide a new and powerful tool for studying the mechanism, diagnosis, and treatment of GC. In this study, we aimed to investigate the role and mechanism of miR-128b in the development and progression of GC. Quantitative real-time PCR (qRT-PCR) was used to measure the expression level of miR-128b in GC tissues and cell lines. We found that miR-128b was significantly down-regulated in GC tissues and cell lines. In addition, over-expression of miR-128b inhibited GC cell proliferation, migration and invasion of GC cells in vitro. Gain-of-function in vitro experiments further showed that the miR-128b mimic significantly promoted GC cell apoptosis. Subsequent dual-luciferase reporter assay identified one of the proto-oncogene A2bR as direct target of miR-128b. Therefore, our results indicate that miR-128b is a proto-oncogene miRNA that can suppresses GC proliferation and migration through down-regulation of the oncogene gene A2bR. Taken together, our results indicate that miR-128b could serve as a potential diagnostic biomarker and therapeutic option for human GC in the near future. (C) 2015 Elsevier Inc. All rights reserved.