Fluorescence-Guided Surgery of Liver Metastasis in Orthotopic Nude-Mouse Models.
Fluorescence-Guided Surgery of Liver Metastasis in Orthotopic Nude-Mouse Models.
复制标题
原位裸小鼠模型中肝转移的荧光引导手术。
DOI：
10.1371/journal.pone.0138752
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hoffman RM
中科院分区：
文献类型：
--
作者：
Murakami T;Hiroshima Y;Zhang Y;Chishima T;Tanaka K;Bouvet M;Endo I;Hoffman RM
我们在此报告荧光引导下肝转移手术的发展。将表达绿色荧光蛋白（GFP）的HT29人结肠癌细胞初步注入裸鼠脾脏。3周后，取已建立的肝转移灶，植入其他裸鼠肝脏左叶，制备原位肝转移模型。14只肝转移小鼠随机分为强光手术组（BLS）和荧光引导手术组（FGS）。7只小鼠用BLS治疗，7只小鼠用FGS治疗。植入三周后，通过腹部正中切口暴露肝脏左叶并有单一转移灶。白光下行BLS。FGS采用手持式便携式荧光成像系统（Dino-Lite）进行。术后残余肿瘤荧光用OV100小动物成像系统显示。用OV100高倍镜清晰显示BLS后残留的肿瘤荧光。相比之下，即使在OV100的高倍倍率下，FGS后残留的肿瘤荧光也未被检测到。这些结果证明了FGS在肝转移中的可行性。
We report here the development of fluorescence-guided surgery of liver metastasis. HT29 human colon cancer cells expressing green fluorescent protein (GFP) were initially injected in the spleen of nude mice. Three weeks later, established liver metastases were harvested and implanted on the left lobe of the liver in other nude mice in order to make an orthotopic liver metastasis model. Fourteen mice with a single liver metastasis were randomized into bright-light surgery (BLS) or fluorescence-guided surgery (FGS) groups. Seven mice were treated with BLS, seven were treated with FGS. Three weeks after implantation, the left lobe of the liver with a single metastasis was exposed through a median abdominal incision. BLS was performed under white light. FGS was performed using a hand-held portable fluorescence imaging system (Dino-Lite). Post-surgical residual tumor fluorescence was visualized with the OV100 Small Animal Imaging System. Residual tumor fluorescence after BLS was clearly visualized at high magnification with the OV100. In contrast, residual tumor fluorescence after FGS was not detected even at high magnification with the OV100. These results demonstrate the feasibility of FGS for liver metastasis.