In situ elasticity modulation with dynamic substrates to direct cell phenotype.
In situ elasticity modulation with dynamic substrates to direct cell phenotype.
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DOI：
10.1016/j.biomaterials.2009.09.025
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发表时间：
2010-01
期刊：
影响因子：
14
通讯作者：
Anseth, Kristi S.
中科院分区：
文献类型：
--
作者：
Kloxin, April M.;Benton, Julie A.;Anseth, Kristi S.
微环境弹性影响关键的细胞功能，如分化、细胞骨架组织和过程延伸。不幸的是，很少有材料允许弹性调制实时探测其对这些动态细胞过程的直接影响。在这里，提出了一种新的方法光化学调制弹性在任何时间点的细胞的微环境。光降解水凝胶在细胞相容条件下辐照和降解，产生类似于软组织的大范围弹性模量，并使用流变学和原子力显微镜（AFM）对其进行表征。探讨弹性模量对瓣膜间质细胞（VIC）活化成肌成纤维细胞的影响。在这些研究中，梯度样品被用来鉴定促进或抑制VIC肌成纤维细胞激活的模组。有了这些知识，vic被培养在高模量，激活水凝胶底物上，独特的是，结果表明，通过照射降低底物模量逆转了这种激活，表明肌成纤维细胞可以通过改变底物的模量来失活。这一发现对于合理设计用于组织再生的生物材料很重要，并为纤维化疾病的进展提供了见解。这些可光降解的水凝胶展示了通过底物弹性的动态变化来探测和指导细胞功能的能力。
Microenvironment elasticity influences critical cell functions such as differentiation, cytoskeletal organization, and process extension. Unfortunately, few materials allow elasticity modulation in real-time to probe its direct effect on these dynamic cellular processes. Here, a new approach is presented for the photochemical modulation of elasticity within the cell's microenvironment at any point in time. A photodegradable hydrogel was irradiated and degraded under cytocompatible conditions to generate a wide range of elastic moduli similar to soft tissues and characterized using rheometry and atomic force microscopy (AFM). The effect of the elastic modulus on valvular interstitial cell (VIC) activation into myofibroblasts was explored. In these studies, gradient samples were used to identify moduli that either promote or suppress VIC myofibroblastic activation. With this knowledge, VICs were cultured on a high modulus, activating hydrogel substrate, and uniquely, results show that decreasing the substrate modulus with irradiation reverses this activation, demonstrating that myofibroblasts can be de-activated solely by changing the modulus of the underlying substrate. This finding is important for the rational design of biomaterials for tissue regeneration and offers insight into fibrotic disease progression. These photodegradable hydrogels demonstrate the capability to both probe and direct cell function through dynamic changes in substrate elasticity.
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影响因子：
6.9
作者：
Cushing, MC;Liao, JT;Anseth, KS
通讯作者：
Anseth, KS
DOI：
10.1083/jcb.200405004
发表时间：
2004-09-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Engler AJ;Griffin MA;Sen S;Bönnemann CG;Sweeney HL;Discher DE
通讯作者：
Discher DE
影响因子：
14
作者：
Peyton, Shelly R.;Raub, Christopher B.;Putnam, Andrew J.
通讯作者：
Putnam, Andrew J.
影响因子：
3.4
作者：
Lo, CM;Wang, HB;Wang, YL
通讯作者：
Wang, YL
DOI：
10.1016/s0022-2828(87)80529-1
发表时间：
1987-12-01
影响因子：
5
作者：
JOHNSON, CM;HANSON, MN;HELGESON, SC
通讯作者：
HELGESON, SC