Characterization of 155 EST-derived microsatellites from Atlantic cod (Gadus morhua) and validation for linkage mapping
Characterization of 155 EST-derived microsatellites from Atlantic cod (Gadus morhua) and validation for linkage mapping
复制标题
DOI：
10.1111/j.1755-0998.2008.02475.x
复制
发表时间：
2009-05-01
影响因子：
7.7
通讯作者：
Bowman, S.
中科院分区：
文献类型：
--
作者：
Higgins, B.;Hubert, S.;Bowman, S.
微卫星标记大西洋鳕鱼（Gadus morhua）的30 630表达的序列标签的集合中确定。为395个微卫星设计引物，成功扩增出155个微卫星。等位基因数为1 ~ 26个，平均6.34个。平均观察和预期杂合性分别为0.50和0.54。一个子集的105个微卫星检测孟德尔分离没有显着的分离时，纠正多次测试失真。在4个位点检测到等位基因。发现23个位点的显著blastx匹配。这些微卫星将用于创建连锁图，以加强商业鳕鱼育种的遗传选择。
Microsatellite markers for Atlantic cod (Gadus morhua) were identified from a collection of 30 630 expressed sequence tags. Primers were designed for 395 microsatellites and 155 were successfully amplified. Allele number varied from 1 to 26 (average 6.34). Average observed and expected heterozygosities were 0.50 and 0.54, respectively. A subset of 105 microsatellites tested for Mendelian segregation showed no significant distortion of segregation when correcting for multiple tests. Null alleles were detected at four loci. Significant blastx matches were found for 23 loci. These microsatellites will be used to create a linkage map to enhance genetic selection in commercial cod breeding.