Antimicrobial Activity of Nitric Oxide-Releasing Ti-6Al-4V Metal Oxide.
Antimicrobial Activity of Nitric Oxide-Releasing Ti-6Al-4V Metal Oxide.
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DOI：
10.3390/jfb8020020
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发表时间：
2017-06-21
影响因子：
4.8
通讯作者：
Gawalt ES
中科院分区：
文献类型：
--
作者：
Reger NA;Meng WS;Gawalt ES
钛及钛合金材料由于材料强度高，常用于关节置换。病原微生物很容易粘附在金属植入物的表面，导致植入物失败的可能性增加。钛铝钒 (Ti-6Al-4V) 金属氧化物植入材料的表面经过功能化，可传递小抗菌分子一氧化氮。 S-亚硝基青霉胺是一种 S-亚硝基硫醇一氧化氮供体，利用自组装单层共价固定在金属氧化物表面。红外光谱用于确认 S-亚硝基硫醇供体与 Ti-Al-4V 表面的附着。在生理条件下，S-亚硝基青霉胺的附着导致一氧化氮 (NO) 释放量为 89.6 ± 4.8 nmol/cm2。这种低浓度的一氧化氮使大肠杆菌和表皮葡萄球菌的生长分别减少了 41.5 ± 1.2% 和 25.3 ± 0.6%。将释放 Ti-6Al-4V 的 S-亚硝基硫醇与常用抗生素四环素结合使用，可将抗生素的有效性提高 35.4 ± 1.3%，从而可以使用较低剂量的抗生素。没有观察到氨苄西林与 S-亚硝基青霉胺修饰的 Ti-6Al-4V 对表皮葡萄球菌的协同作用。功能化的 Ti-6Al-4V 表面对小鼠成纤维细胞没有细胞毒性。
Titanium and titanium alloy materials are commonly used in joint replacements, due to the high strength of the materials. Pathogenic microorganisms can easily adhere to the surface of the metal implant, leading to an increased potential for implant failure. The surface of a titanium-aluminum-vanadium (Ti-6Al-4V) metal oxide implant material was functionalized to deliver an small antibacterial molecule, nitric oxide. S-nitroso-penicillamine, a S-nitrosothiol nitric oxide donor, was covalently immobilized on the metal oxide surface using self-assembled monolayers. Infrared spectroscopy was used to confirm the attachment of the S-nitrosothiol donor to the Ti-Al-4V surface. Attachment of S-nitroso-penicillamine resulted in a nitric oxide (NO) release of 89.6 ± 4.8 nmol/cm2 under physiological conditions. This low concentration of nitric oxide reduced Escherichia coli and Staphylococcus epidermidis growth by 41.5 ± 1.2% and 25.3 ± 0.6%, respectively. Combining the S-nitrosothiol releasing Ti-6Al-4V with tetracycline, a commonly-prescribed antibiotic, increased the effectiveness of the antibiotic by 35.4 ± 1.3%, which allows for lower doses of antibiotics to be used. A synergistic effect of ampicillin with S-nitroso-penicillamine-modified Ti-6Al-4V against S. epidermidis was not observed. The functionalized Ti-6Al-4V surface was not cytotoxic to mouse fibroblasts.