Characterization of multinucleated giant cells in synovium and subchondral bone in knee osteoarthritis and rheumatoid arthritis.
Characterization of multinucleated giant cells in synovium and subchondral bone in knee osteoarthritis and rheumatoid arthritis.
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DOI：
10.1186/s12891-015-0664-5
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发表时间：
2015-08-27
影响因子：
2.3
通讯作者：
Walsh DA
中科院分区：
文献类型：
--
作者：
Prieto-Potin I;Largo R;Roman-Blas JA;Herrero-Beaumont G;Walsh DA
多核巨细胞自从在类风湿性关节炎滑膜中首次被发现以来，就在各种关节炎中被发现。然而，它们在骨关节炎（OA）或类风湿性关节炎（RA）发病机制中的作用仍然广泛未知。我们的目的是研究多核巨细胞（MGC）在OA或RA患者滑膜和软骨下骨组织中的存在和特征。膝关节滑膜和软骨下骨样本来自年龄匹配的接受OA或RA全关节置换术的患者，或非关节炎死后（PM）对照组。使用索引组织切片，通过组织学炎症等级对OA滑膜进行分层。滑膜炎采用Krenn评分。组织学研究采用针对巨噬细胞标记物或组织蛋白酶K的特异性抗体，或TRAP酶测定。发炎的OA和RA滑膜比非发炎的OA和PM滑膜显示更多的多核巨细胞。MGC数量与滑膜炎严重程度之间存在显著相关性。TRAP阴性/组织蛋白酶K阴性Langhans样亚型在OA中占主导地位，而Langhans样和TRAP阳性/组织蛋白酶K阴性异物样亚型在RA中最常见。在OA和RA滑膜中也观察到血浆样和泡沫样亚型，后者发现于脂肪细胞周围。TRAP阳性/组织蛋白酶K阳性破骨细胞仅在邻近软骨下骨表面被识别。TRAP阳性破骨细胞在OA和RA中的软骨下骨与PM对照相比显著增加。多核巨细胞与滑膜炎的严重程度相关，软骨下破骨细胞数量在OA和RA中增加。针对多核巨细胞的进一步研究是必要的，以阐明它们对关节炎相关症状和关节损伤的贡献。本文的在线版本（doi：10.1186/s12891-015-0664-5）包含补充材料，可供授权用户使用。
Multinucleated giant cells have been noticed in diverse arthritic conditions since their first description in rheumatoid synovium. However, their role in the pathogenesis of osteoarthritis (OA) or rheumatoid arthritis (RA) still remains broadly unknown. We aimed to study the presence and characteristics of multinucleated giant cells (MGC) both in synovium and in subchondral bone tissues of patients with OA or RA. Knee synovial and subchondral bone samples were from age-matched patients undergoing total joint replacement for OA or RA, or non-arthritic post mortem (PM) controls. OA synovium was stratified by histological inflammation grade using index tissue sections. Synovitis was assessed by Krenn score. Histological studies employed specific antibodies against macrophage markers or cathepsin K, or TRAP enzymatic assay. Inflamed OA and RA synovia displayed more multinucleated giant cells than did non-inflamed OA and PM synovia. There was a significant association between MGC numbers and synovitis severity. A TRAP negative/cathepsin K negative Langhans-like subtype was predominant in OA, whereas both Langhans-like and TRAP-positive/cathepsin K-negative foreign-body-like subtypes were most commonly detected in RA. Plasma-like and foam-like subtypes also were observed in OA and RA synovia, and the latter was found surrounding adipocytes. TRAP positive/cathepsin K positive osteoclasts were only identified adjacent to subchondral bone surfaces. TRAP positive osteoclasts were significantly increased in subchondral bone in OA and RA compared to PM controls. Multinucleated giant cells are associated with synovitis severity, and subchondral osteoclast numbers are increased in OA, as well as in RA. Further research targeting multinucleated giant cells is warranted to elucidate their contributions to the symptoms and joint damage associated with arthritis. The online version of this article (doi:10.1186/s12891-015-0664-5) contains supplementary material, which is available to authorized users.