TrnL–trnF Intergenic Spacer and trnL Intron Define Major Clades Within Luzula and Juncus (Juncaceae): Importance of Structural Mutations
TrnL–trnF Intergenic Spacer and trnL Intron Define Major Clades Within Luzula and Juncus (Juncaceae): Importance of Structural Mutations
复制标题
DOI：
10.1007/s00239-004-2598-7
复制
发表时间：
2004-07
影响因子：
3.9
通讯作者：
L. Drábková;J. Kirschner;Č. Vlček;V. Paces
中科院分区：
文献类型：
--
作者：
L. Drábková;J. Kirschner;Č. Vlček;V. Paces
对 55 个灯芯草科类群（灯芯草、Luzula、Rostkovia 和 Oxychloë）叶绿体 DNA 的 trnL 内含子和 trnL-trnF 基因间间隔区的 752 至 1,097 个碱基对进行了测序。叶绿体trnL-trnF区域内的17个结构突变（标记为A到M的13个插入缺失，trnF假基因的3个部分，以及假基因中的插入“o”）被检查为灯芯草科系统发育关系的可能指标。三裂灯芯草（Steirochloa节）通过两个大（> 80 bp）插入缺失与其他类群明显分开。 “南半球进化枝”得到了 trnL 内含子中独特插入（334 bp）的有力支持。 Luzulaw 的单系性由 trnL-F 间隔区中的三个小 (<10 bp) 插入缺失支持。在代表 Luzula 属分类学和地理多样性的所有 22 个检查成员中都发现了它们。在灯心草亚属灯心草属中发现了串联重复的 tRNA 假基因，并且也由四个小的独特插入缺失支持。受体茎和 D 结构域编码区由独特的 8 bp 插入分隔开。在假基因重复中未发现 T 结构域和受体茎编码区。只有灯心草部分 Ozophyllum 和 Iridifolii 含有 tRNAF 编码 DNA 的 5' 受体茎、D 结构域和反密码子结构域。 trnL 内含子和 trnL-trnF 基因间隔区的结构突变对于灯芯草科的系统发育重建很有用。
Seven hundred fifty-two to one thousand ninety-seven base pairs of thetrnL intron andtrnL–trnF intergenic spacer of the chloroplast DNA of 55 Juncaceae taxa (Juncus,Luzula,Rostkovia, andOxychloë) was sequenced. Seventeen structural mutations (13 indels marked A to M, 3 parts of thetrnF pseudogene, and insertion “o” within a pseudogene) within the chloroplasttrnL–trnF region were examined as possible indicators for phylogenetic relationships in Juncaceae.Juncus trifidus(section Steirochloa) was clearly separated from the other taxa by two large (>80 bp) indels. The “Southern Hemisphere clade” was strongly supported by a unique insertion (334 bp) in thetrnL intron. The monophyly ofLuzulawas supported by three small (<10 bp) indels in thetrnL-F spacer. They were found in all 22 examined members that represent the taxonomic and geographical diversity of the genusLuzula. A tandemly duplicated tRNA pseudogene was found in theJuncussubgenusJuncusspecies and is supported by four small unique indels too. The acceptor stem and D-domain-encoding regions are separated by a unique 8-bp insertion. The T-domain and acceptor stem-encoding regions were not found in the pseudogene repeats. Only theJuncussections Ozophyllum and Iridifolii contain the 5′ acceptor stem, D-domain, and anticodon domain of the tRNAF encoding DNA. The structural mutations in thetrnL intron and thetrnL–trnF intergenic spacer are useful for phylogenetic reconstruction in the Juncaceae.