In vitro biosynthesis of oestradiol-17β and 17α,20gb-dihydroxy-4-pregnen-3-one by vitellogenic ovarian follicles from migrating New Zealand longfinned eels (Anguilla dieffenbachii)
In vitro biosynthesis of oestradiol-17β and 17α,20gb-dihydroxy-4-pregnen-3-one by vitellogenic ovarian follicles from migrating New Zealand longfinned eels (Anguilla dieffenbachii)
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新西兰长鳍鳗鲡（Anguilla dieffenbachii）卵黄发生卵泡体外生物合成雌二醇-17β和17α,20gb-二羟基-4-pregnen-3-one
DOI：
10.1016/0044-8486(95)01008-4
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发表时间：
1995
期刊：
影响因子：
4.5
通讯作者：
G. Young
中科院分区：
文献类型：
--
作者：
P. Lokman;G. Young
研究了自然产卵洄游期采集的迪芬巴赫鳗鲡（Anguilladieffenbachii）卵黄发生卵巢组织体外分泌17β-雌二醇（E2）和17α，20 β-二羟基-4-异戊烯-3-酮（17，20-DHP）的能力。在存在或不存在孕烯醇酮、雄烯二酮或睾酮的情况下，将卵巢碎片与或不与人绒毛膜促性腺激素（hCG）一起孵育。尽管体内E2水平相对较高（2.48 ± 0.28 ng ml−1，n = 7），但体外e2产量较低。相比之下，17，20-DHP在体内的水平很低（低于0.16 ng ml−1），而在体外，在存在双烯醇酮的情况下，产量很高，表明卵黄发生卵泡中有很强的20β-羟基类固醇脱氢酶活性。在任何孵育中，hCG的加入均未显著刺激E2或17，20-DHP的产生高于对照组。这些结果与鲑科动物中类似研究的结果相反，表明鲑科动物卵泡控制E2和17，20-DHP产生的模型可能不完全适用于鳗鱼卵泡。
In vitro oestradiol-17β (E2) and 17α,20β-dihydroxy-4-pregnen-3-one (17,20-DHP) production by vitellogenic ovarian tissue from Anguilla dieffenbachii captured during the natural spawning migration was examined. Ovarian fragments were incubated with or without human chorionic gonadotropin (hCG) in the presence or absence of pregnenolone, androstenedione or testosterone. Despite relatively high E2levels in vivo (2.48 ± 0.28 ng ml−1, n = 7), in vitro e2production was low. In contrast, 17, 20-DHP levels were low in vivo (less than 0.16 ng ml−1), while production was high in the presence of pregnenolone in vitro, indicating strong 20β-hydroxysteroid dehydrogenase activity in vitellogenic follicles. Addition of hCG did not significantly stimulate E2or 17,20-DHP production above that of controls in any of the incubations. These results are in contrast to those arising from similar studies in salmonids and indicate that the models developed for control of E2and 17,20-DHP production by the salmonid follicle may not be fully applicable to the eel follicle.