Mapping QTLs for root traits under different nitrate levels at the seedling stage in maize (Zea mays L.)
Mapping QTLs for root traits under different nitrate levels at the seedling stage in maize (Zea mays L.)
复制标题
玉米苗期不同硝酸盐水平下根系性状的 QTL 定位 (Zea mays L.)
DOI：
10.1007/s11104-008-9562-z
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发表时间：
2008-04-01
期刊：
影响因子：
4.9
通讯作者：
Mi, Guohua
中科院分区：
文献类型：
--
作者：
Liu, Jianchao;Li, Jiansheng;Mi, Guohua
氮素流失是世界性的作物生产问题。除合理施氮外，培育氮高效品种是另一条途径。根系构型是影响氮素吸收的重要因素。然而，很少有人知道的分子遗传基础根生长与氮供应。本研究以F8代玉米（Zea may L.）以94个株系组成的重组自交系群体为材料，研究了不同硝酸盐水平下根系性状的QTL定位。在低氮和高氮条件下，研究了小麦侧根长（LRL）、侧根长（ARL）、最大侧根长（MARL）、侧根数（ARN）和平均侧根长（AARL）的变化。共检测到17个QTL，其中14个与已发表的根系性状QTL位于同一染色体区域。位于第1染色体上的一个主效QTL（位于bnlg1025和umc2029之间）可以解释43.7%的表型变异。该QTL与先前报道的与根系性状、籽粒产量和氮吸收相关的QTL共定位。研究结果表明，长轴根对氮高效吸收具有重要作用，AARL主效QTL可作为氮高效玉米基因型选育的标记。
Nitrogen (N) loss is a worldwide problem in crop production. Apart from reasonable N fertilizer application, breeding N efficient cultivars provides an alternative way. Root architecture is an important factor determining N acquisition. However, little is known about the molecular genetic basis for root growth in relation to N supply. In the present study, an F8 maize (Zea may L.) recombinant inbred (RI) population consisting of 94 lines was used to identify the QTLs for root traits under different nitrate levels. The lateral root length (LRL), axial root length (ARL), maximal axial root length (MARL), axial root number (ARN) and average axial root length (AARL) were evaluated under low N (LN) and high N (HN) conditions in a hydroponics system. A total of 17 QTLs were detected among which 14 loci are located on the same chromosome region as published QTLs for root traits. A major QTL on chromosome 1 (between bnlg1025 and umc2029) for the AARL under LN could explain 43.7% of the phenotypic variation. This QTL co-localizes with previously reported QTLs that associate with root traits, grain yield, and N uptake. Our results indicate that longer axial roots are important for efficient N acquisition and the major QTL for AARL may be used as a marker in breeding N efficient maize genotypes.