POSITION-RELATED CAPACITY FOR DIFFERENTIATION OF LIMB MESENCHYME IN CELL-CULTURE
POSITION-RELATED CAPACITY FOR DIFFERENTIATION OF LIMB MESENCHYME IN CELL-CULTURE
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DOI：
10.1016/0012-1606(79)90303-8
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发表时间：
1979-01-01
影响因子：
2.7
通讯作者：
SINGLEY, CT
中科院分区：
文献类型：
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作者：
AHRENS, PB;SOLURSH, M;SINGLEY, CT
定量比较（即，软骨结节数）在用来自软骨形成前鸡翅芽内不同位置（核心与周边）的细胞制备的微团细胞培养物之间进行软骨分化。在微团培养中，翅芽核心细胞比来自相同肢体的外周细胞表现出更大的向软骨分化的发育偏好。成肌细胞出现更频繁地从翅芽周边比在那些从核心组织制备的文化。23-24期的翅芽显然不是一个同质的多能间充质细胞群。相反，23-24期的翅芽包含2类细胞，每一类细胞的特征在于偏向软骨或肌肉分化，以及第3类未表征的间充质细胞。
A quantitative comparison (i.e., number of cartilage nodules) of cartilage differentiation was made between micromass cell cultures prepared with cells from different locations (core vs. periphery) within prechondrogenic chick wing buds. Wing bud core cells in micromass culture exhibit a greater development bias toward cartilage differentiation than periphery cells from the same limbs. Myogenic cells appear more frequently in cultures prepared from wing bud periphery than in those prepared from core tissue. A stage 23-24 wing bud apparently is not a homogeneous population of multipotential mesenchymal cells. Instead, a stage 23-24 wing bud contains 2 classes of cells, each characterized by a bias for either cartilage or muscle differentiation, and a 3rd class of uncharacterized mesenchymal cells.