Multi-walled carbon nanotube interactions with human epidermal keratinocytes
Multi-walled carbon nanotube interactions with human epidermal keratinocytes
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DOI：
10.1016/j.toxlet.2004.11.004
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发表时间：
2005-03-15
影响因子：
3.5
通讯作者：
Riviere, JE
中科院分区：
文献类型：
--
作者：
Monteiro-Riviere, NA;Nemanich, RJ;Riviere, JE
碳纳米管在多个工程学科中有着广泛的应用。然而，人们对这些颗粒的毒性或与细胞的相互作用知之甚少。利用微波等离子体增强化学气相沉积系统制备了碳纳米管薄膜。将人表皮角质形成细胞(HEK)分别暴露于0.1、0.2和0.4 mg/ml的多壁碳纳米管(MWCNT)中1、2、4、8、12、24和48h，用透射电子显微镜观察HEK中是否存在MWCNT。在此，我们报告了在所有时间点，未经化学修饰的多壁碳纳米管存在于HEK的细胞质空泡中。MWCNT还以时间依赖的方式诱导HEKS释放促炎细胞因子IL-8。这些数据清楚地表明，MWCNT既不是衍生的，也不是针对生物应用进行优化的，它既能够定位于目标上皮细胞，也能够在目标上皮细胞中启动刺激反应，这构成了制造的纳米管职业暴露的主要途径。(C)2004爱思唯尔爱尔兰有限公司。保留所有权利。
Carbon nanotubes have widespread applications in multiple engineering disciplines. However, little is known about the toxicity or interaction of these particles with cells. Carbon nanotube films were grown using a microwave plasma enhanced chemical vapor deposition system. Human epidermal keratinocytes (HEK) were exposed to 0.1, 0.2, and 0.4 mg/ml of multi-walled carbon nanotubes (MWCNT) for 1, 2, 4, 8, 12, 24 and 48 h. HEK were examined by transmission electron microscopy for the presence of MWCNT. Here we report that chemically unmodified MWCNT were present within cytoplasmic vacuoles of the HEK at all time points. The MWCNT also induced the release of the proinflammatory cytokine interleukin 8 from HEKs in a time dependent manner. These data clearly show that MWCNT, not derivatized nor optimized for biological applications, are capable of both localizing within and initiating an irritation response in a target epithelial cell that composes a primary route of occupational exposure for manufactured nanotubes. (C) 2004 Elsevier Ireland Ltd. All rights reserved.