Mycobacterium-mediated chemokine expression in pleural mesothelial cells:: Role of C-C chemokines in tuberculous pleurisy
Mycobacterium-mediated chemokine expression in pleural mesothelial cells:: Role of C-C chemokines in tuberculous pleurisy
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DOI：
10.1086/314442
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发表时间：
1998-11-01
影响因子：
6.4
通讯作者：
Antony, VB
中科院分区：
文献类型：
--
作者：
Mohammed, KA;Nasreen, N;Antony, VB
肺结核的特征是肉芽肿性炎症并伴有单核吞噬细胞广泛浸润，但吞噬细胞募集至胸膜腔的机制尚不清楚。在这项研究中，与充血性心力衰竭患者的胸腔积液相比，结核病患者的胸腔积液中含有显着 (P < .001) 更多的生物活性 MIP-1 α 和 MCP-1（C-C 细胞因子）。通过免疫细胞化学方法在结核性胸膜炎患者的胸膜活检切片中检测到抗原性 MIP-1 α 和 MCP-1。在体外，用卡介苗（BCC）或干扰素（IFN）-γ刺激胸膜间皮细胞产生MIP-1α和MCP-1，逆转录聚合酶链反应研究证实BCG和IFN-γ均诱导间皮细胞中MIP-1α和MCP-1表达，证明间皮细胞来源的C-C趋化因子在将单核细胞募集至胸膜腔。
Pulmonary tuberculosis is characterized by granulomatous inflammation with an extensive infiltration of mononuclear phagocytes, but the mechanisms of phagocyte recruitment to the pleural space is unknown. In this study, pleural fluid from patients with tuberculosis contained significantly (P < .001) more biologically active MIP-1 alpha and MCP-1 (C-C cytokines) than did effusions from patients with congestive heart failure. Antigenic MIP-1 alpha and MCP-1 was detected by immunocytochemistry in pleural biopsy sections of patients with tuberculous pleurisy. In vitro, pleural mesothelial cells stimulated with bacille Calmette-Guerin (BCC) or interferon (IFN)-gamma produced MIP-1 alpha and MCP-1, Reverse transcription-polymerase chain reaction studies confirmed that both BCG and IFN-gamma induced MIP-1 alpha and MCP-1 expression in mesothelial cells, demonstrating that mesothelial cell-derived C-C chemokines play a biologically important role in the recruitment of mononuclear cells to the pleural space.