Analysis of gene transcription in bovine nuclear transfer embryos reconstructed with granulosa cell nuclei
Analysis of gene transcription in bovine nuclear transfer embryos reconstructed with granulosa cell nuclei
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DOI：
10.1095/biolreprod63.4.1034
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发表时间：
2000-10-01
影响因子：
3.6
通讯作者：
Trounson, AO
中科院分区：
文献类型：
--
作者：
Daniels, R;Hall, V;Trounson, AO
使用体细胞核移植程序的动物生产的低效率被认为是供体体细胞核的不完全重编程的结果，这导致发育重要基因的缺乏或异常表达。然而，我们目前对体细胞核重编程过程及其对基因表达的影响的理解是有限的。在这项研究中，我们比较了6个发育重要的基因，Oct4，IL 6，FGF2，FGF4，FGFR2和gp130在单个体外受精（IVF）和核移植胚胎重建使用颗粒细胞的供体核的转录模式。在植入前发育阶段，在IVF和核移植胚胎中检测到Oct4、FGF2和gp130的转录模式相似。然而，一些桑椹胚和囊胚期胚胎来自核移植程序显示异常转录的IL 6，FGF4和FGFr 2。先前的研究表明，这三个基因在小鼠的着床、早期着床后发育或两者中起重要作用。因此，在核移植胚胎中检测到的异常转录模式可能导致胚胎存活率降低。
The low efficiency of animal production using somatic cell nuclear transfer procedures is considered to be the result of an incomplete reprogramming of the donor somatic cell nucleus, which leads to a lack of, or abnormal expression of developmentally important genes. However, our current understanding of the process of somatic cell nuclear reprogramming and its effect on gene expression is limited. In this study, we compare the transcription patterns of six developmentally important genes, Oct4, IL6, FGF2, FGF4, FGFr2, and gp130 in single in vitro fertilized (IVF) and nuclear transfer embryos reconstructed using granulosa cells for the donor nuclei. Similar patterns of transcription were detected for Oct4, FGF2, and gp130 in IVF and nuclear transfer embryos during the preimplantation stages of development. However, a number of morula- and blastocyst-stage embryos derived from nuclear transfer procedures showed abnormal transcription of IL6, FGF4, and FGFr2. Previous studies have demonstrated that these three genes play an important role in implantation, early postimplantation development, or both in the mouse. Therefore, the aberrant transcription patterns detected in nuclear transfer embryos may lead to a reduction in embryo viability.