Carbon paste-based electrochemical detectors for microchip capillary electrophoresis/electrochemistry
Carbon paste-based electrochemical detectors for microchip capillary electrophoresis/electrochemistry
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DOI：
10.1039/b009827m
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发表时间：
2001-01-01
期刊：
影响因子：
4.2
通讯作者：
Lunte, SM
中科院分区：
文献类型：
--
作者：
Martin, RS;Gawron, AJ;Lunte, SM
首次报道了碳糊电化学检测器在微芯片毛细管电泳（CE）中的应用。通过将含有分离和注入通道的PDMS层可逆地密封到含有碳糊工作电极的单独的PDMS层来构建基于聚（二甲基硅氧烷）（PDMS）的微芯片CE装置。采用单电极端通道安培检测法对萘二甲醛衍生化的氨基酸进行检测。串联放置两个电极以进行双电极检测。这种方法被证明用于检测铜（II）肽复合物。碳糊的一个主要优点是催化剂可以很容易地结合到电极中。用酞菁钴化学改性的碳糊用于在CE分离之后检测硫醇。这些设备说明了一个容易构建的微芯片CE系统与碳基检测器，表现出可调的选择性的潜力。
The first reported use of a carbon paste electrochemical detector for microchip capillary electrophoresis (CE) is described. Poly(dimethylsiloxane) (PDMS)-based microchip CE devices were constructed by reversibly sealing a PDMS layer containing separation and injection channels to a separate PDMS layer that contained carbon paste working electrodes. End-channel amperometric detection with a single electrode was used to detect amino acids derivatized with naphthalene dicarboxaldehyde. Two electrodes were placed in series for dual electrode detection. This approach was demonstrated for the detection of copper(II) peptide complexes. A major advantage of carbon paste is that catalysts can be easily incorporated into the electrode. Carbon paste that was chemically modified with cobalt phthalocyanine was used for the detection of thiols following a CE separation. These devices illustrate the potential for an easily constructed microchip CE system with a carbon-based detector that exhibits adjustable selectivity.