Towards high resolution analysis of metabolic flux in cells and tissues.
Towards high resolution analysis of metabolic flux in cells and tissues.
复制标题
致力于细胞和组织代谢流的高分辨率分析。
DOI：
10.1016/j.copbio.2013.07.001
复制
发表时间：
2013
影响因子：
7.7
通讯作者：
Lee,Kyongbum
中科院分区：
文献类型：
--
作者：
Sims,JamesK;Manteiga,Sara;Lee,Kyongbum
细胞的代谢状态是细胞表型的重要决定因素。目前的全球代谢通量量化方法表征了整体行为。基因组规模模型为涉及多种细胞类型的分析提供了希望。代谢成像的进步可以提供时空分辨分析。新陈代谢从营养物质中提取化学能，利用这些能量形成生物合成的基石，并在协调细胞途径活动的各种小分子之间进行相互转化。细胞的代谢状态不仅决定代谢活跃细胞类型（如肝脏、肌肉和脂肪）的表型，还决定其他特殊细胞类型（如神经元和免疫细胞）的表型。这篇综述的重点是量化细胞内反应通量作为细胞代谢活动的测量方法，重点是涉及哺乳动物组织细胞的研究。强调了未来发展的两个关键领域，单细胞代谢组学和无创成像，这可以实现时空分辨分析，从而克服异质性问题，这是组织代谢的一个显着特征
HighlightsThe metabolic state of a cell is an important determinant of cellular phenotype.Current methods for global metabolic flux quantification characterize bulk behavior.Genome-scale models offer promise for analyses involving multiple cell types.Advances in metabolic imaging could afford spatiotemporally resolved analysis.Metabolism extracts chemical energy from nutrients, uses this energy to form building blocks for biosynthesis, and interconverts between various small molecules that coordinate the activities of cellular pathways. The metabolic state of a cell is increasingly recognized to determine the phenotype of not only metabolically active cell types such as liver, muscle, and adipose, but also other specialized cell types such as neurons and immune cells. This review focuses on methods to quantify intracellular reaction flux as a measure of cellular metabolic activity, with emphasis on studies involving cells of mammalian tissue. Two key areas are highlighted for future development, single cell metabolomics and noninvasive imaging, which could enable spatiotemporally resolved analysis and thereby overcome issues of heterogeneity, a distinctive feature of tissue metabolism