Genetic control of broad-spectrum resistance to turnip mosaic virus in Brassica rapa (Chinese cabbage)
Genetic control of broad-spectrum resistance to turnip mosaic virus in Brassica rapa (Chinese cabbage)
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DOI：
10.1099/vir.0.83194-0
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发表时间：
2007-11-01
影响因子：
3.8
通讯作者：
Lydiate, Derek J.
中科院分区：
文献类型：
--
作者：
Rusholme, Rachel L.;Higgins, Erin E.;Lydiate, Derek J.
芜菁系 RLR22 对八种不同的芜菁花叶病毒 (TuMV) 分离株具有抗性。建立了基于120个第一回交(B-1)个体中分离的213个标记基因座的白菜遗传图谱，并使用一些RFLP探针与白菜基因组参考图谱进行比对。 B，个体自花授粉产生B1S1，家庭。控制 TuMV 分离株 CDN 1 抗性的两个基因座的存在是根据 B1S 家族中抗性和易感性分离模式的对比确定的。第一个基因，隐性 TuMV 抗性 01 (retr01)，具有隐性抗性等位基因，位于染色体 R4 的上部，并且是第二个基因的上位性。第二个基因是条件性 TuMV 抗性 01 (ConTR01)，具有显性抗性等位基因，位于 R8 染色体的上部。这些基因还控制对 TuMV 分离株 CZE 1 的抗性，并且可能足以解释 RLR22 的广谱抗性。显性抗性基因ConTR01与白菜的三个真核起始因子4E (el/F4E)基因座之一以及可能的elF(iso)4E基因座之一一致。隐性抗性基因retr01显然与甘蓝型油菜A基因组中的elF(iso)4E的三个基因座之一一致，因此，通过推断，与白菜基因组中的elF(iso)4E基因座之一一致。这表明抗性的作用模式是基于拒绝病毒RNA进入植物宿主的翻译起始复合体。基因 retr01 是第一个报道的在芸苔属物种中定位的隐性抗性基因的例子。
The Brassica rapa line RLR22 was resistant to eight diverse turnip mosaic virus (TuMV) isolates. A B. rapa genetic map based on 213 marker loci segregating in 120 first back-cross (B-l) individuals was established and aligned with the B. rapa genome reference map using some of the RFLP probes. B, individuals were self-pollinated to produce B1S1, families. The existence of two loci controlling resistance to TuMV isolate CDN 1 was established from contrasting patterns of segregation for resistance and susceptibility in the B1S, families. The first gene, recessive TuMV resistance 01 (retr01), had a recessive allele for resistance, was located on the upper portion of chromosome R4 and was epistatic to the second gene. The second gene, Conditional TuMV resistance 01 (ConTR01), possessed a dominant allele for resistance and was located on the upper portion of chromosome R8. These genes also controlled resistance to TuMV isolate CZE 1 and might be sufficient to explain the broad-spectrum resistance of RLR22. The dominant resistance gene, ConTR01, was coincident with one of the three eukaryotic initiation factor 4E (el/F4E) loci of B. rapa and possibly one of the loci of elF(iso)4E. The recessive resistance gene retr01 was apparently coincident with one of the three loci of elF(iso)4E in the A genome of Brassica napus and therefore, by inference, in the B. rapa genome. This suggested a mode of action for the resistance that is based on denying the viral RNA access to the translation initiation complex of the plant host. The gene retr01 is the first reported example of a recessive resistance gene mapped in a Brassica species.