Rice miR394 suppresses leaf inclination through targeting an F-box gene, LEAF INCLINATION 4.
Rice miR394 suppresses leaf inclination through targeting an F-box gene, LEAF INCLINATION 4.
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DOI：
10.1111/jipb.12713
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发表时间：
2018-11
影响因子：
11.4
通讯作者：
Li Qu;Li-Bi Lin;Hong-Wei Xue
中科院分区：
文献类型：
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作者：
Li Qu;Li-Bi Lin;Hong-Wei Xue
水稻叶片倾斜度是一个重要的农艺性状，与植株构型和产量密切相关。鉴定控制叶片倾斜的基因将有助于作物改良。虽然各种因素，包括植物激素生长素和油菜素类固醇，已被证明可以调节叶片联合发展，在调节叶片倾斜的microRNA的作用仍然在很大程度上是未知的。在这里，我们功能性地描述了水稻miR 394及其靶基因，叶倾斜4（LC 4），它编码一个F-box蛋白，在调节叶倾斜中的作用。我们表明，miR 394和LC 4的工作，拮抗，调节叶片联合发展和水稻建筑，通过调节近轴薄壁细胞的扩展和伸长。miR 394的抑制表达或LC 4的增强表达导致叶角增大，而通过CRISPR/Cas9降低LC 4表达导致叶倾斜度降低，表明LC 4作为用于水稻结构改良的候选物。LC 4与SCF E3泛素连接酶复合物的组分SKP 1相互作用，并且miR 394和LC 4的转录都受生长素调节。具有改变的miR 394或LC 4表达的水稻植物具有改变的生长素响应，表明miR 394-LC 4模块介导对于确定水稻叶片倾斜和结构重要的生长素效应。
Rice leaf inclination is an important agronomic trait, closely related to plant architecture and yield. Identification of genes controlling leaf inclination would assist in crop improvement. Although various factors, including the plant hormones auxin and brassinosteroids, have been shown to regulate lamina joint development, the role of microRNAs in regulating leaf inclination remains largely unknown. Here, we functionally characterize the role of rice miR394 and its target, LEAF INCLINCATION 4 (LC4), which encodes an F-box protein, in the regulation of leaf inclination. We show that miR394 and LC4 work, antagonistically, to regulate leaf lamina joint development and rice architecture, by modulating expansion and elongation of adaxial parenchyma cells. Suppressed expression of miR394, or enhanced expression of LC4, results in enlarged leaf angles, whereas reducing LC4 expression by CRISPR/Cas9 leads to reduced leaf inclination, suggesting LC4 as candidate for use in rice architecture improvement. LC4 interacts with SKP1, a component of the SCF E3 ubiquitin ligase complex, and transcription of both miR394 and LC4 are regulated by auxin. Rice plants with altered expression of miR394 or LC4 have altered auxin responses, indicating that the miR394-LC4 module mediates auxin effects important for determining rice leaf inclination and architecture.