Interference by Rheumatoid Factor and Clq in the Detection of IgG Complexes: Studies of Model Systems by ELIS A
Interference by Rheumatoid Factor and Clq in the Detection of IgG Complexes: Studies of Model Systems by ELIS A
复制标题
类风湿因子和 Clq 对 IgG 复合物检测的干扰：通过 ELIS A 对模型系统的研究
DOI：
10.1111/j.1365-3083.1982.tb00655.x
复制
发表时间：
1982
影响因子：
3.7
通讯作者：
P. Lindström
中科院分区：
文献类型：
--
作者：
O. Wager;P. Lindström
通过酶联免疫吸附测定 (ELISA) 研究了纯化的 IgM 类风湿因子 (RF) 和 Clq 对模型复合物检测的干扰。可溶性单克隆和多氯冷球蛋白 IgM RF 和人或猪 Clq 剂量依赖性地抑制人 IgG 复合物与固相 IgM RF、Clq 和牛凝集素 (Kg) 的结合。 Kg 结合的抑制模式取决于反应物之间的对抗顺序。为了实现抑制，Clq 或 RF 必须在用新鲜的正常人血清 (NHS) 处理之前向复合物提供；用新鲜 NHS（alexlated 复合物）预处理的复合物对抑制具有抵抗力。 56°C 或 63°C 加热会破坏 NHS 的排毒能力，但不会影响排毒复合物的 Kg 结合。根据目前和之前的发现，得出的结论是，内在 Clq 和 RF 可能会干扰 Clq 或 RF 结合测定中循环免疫复合物 (CIC) 的检测；它们不太可能干扰 Kg 结合测定中 CIC 的检测。讨论了与选择性去除内在 Clq 和 RF 相关的问题。
Interference by purified IgM rheumaloid factors (RFs) and Clq in the detection of model complexes was studied by enzyme‐linked immunosorbent assay (ELISA). Soluble monoclonal and polycloral cryoglobulin IgM RFs und human or porcine Clq inhibited dosedepcndently the binding of human IgG complexes to solid‐phase IgM RF, Clq, and bovine conglutinin (Kg). The inhibition patterns of the Kg binding were dependent on the order of confrontation between the reactants. To achieve inhibition, Clq or RF had to he offered lo the complexes before treatment with fresh normal human serum (NHS); complexes pretreated with fresh NHS (alexinated complexes) were resistant to inhibition. Heating at 56 °C or 63 °C, which destroyed the alexinating capacity of NHS, did not affect the Kg binding of alexinated complexes. On the basis of present and previous findings it is concluded that intrinsic Clq and RFs are likely to interfere with detection of circulating immune complexes (CICs) in Clq‐ or RF‐binding assays; they are less likely to interfere with detection of CICs in Kg‐binding assays. The problems associated with selective removal of intrinsic Clq and RFs are discussed.