Antisense transcription is coupled to nucleosome occupancy in sense promoters
Antisense transcription is coupled to nucleosome occupancy in sense promoters
复制标题
反义转录与有义启动子中的核小体占据相结合
DOI：
10.1093/bioinformatics/bts534
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发表时间：
2012-11-01
期刊：
影响因子：
5.8
通讯作者：
Dai, Xianhua
中科院分区：
文献类型：
--
作者：
Dai, Zhiming;Dai, Xianhua
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MOTIVATION
Genome-wide pervasive transcription is widespread in eukaryotes, revealing an extensive array of antisense transcription that involves hundreds of previously unknown non-coding RNAs. Individual cases have shown that antisense transcription influences sense transcription, however, genome-wide mechanisms of how antisense transcription regulates sense transcription remain to be elucidated.
RESULTS
Here, we performed a systematic analysis of sense-antisense transcription and nucleosome occupancy in yeast. We found that antisense transcription is associated with nucleosome occupancy in sense promoters. Using RNA polymerase II inactivation data as a reasonable approximation to antisense transcription inactivation data, we further showed that antisense transcripts increase nucleosome occupancy in sense promoter regions they overlap, and reduce nucleosome occupancy in sense promoter regions around their transcription termination sites. These results reveal the previously unappreciated roles of antisense transcription in directing nucleosome occupancy in sense promoters. Our findings will have implications in understanding regulatory functions of antisense transcription.