Geographic differences in paralytic shellfish poisoning toxin profiles among Japanese populations of Alexandrium tamarense and A. catenella (Dinophyceae)
Geographic differences in paralytic shellfish poisoning toxin profiles among Japanese populations of Alexandrium tamarense and A. catenella (Dinophyceae)
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日本塔玛亚历山大藻和链状甲藻（甲藻纲）种群麻痹性贝类中毒毒素谱的地理差异
DOI：
10.1046/j.1440-1835.2001.00215.x
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发表时间：
2001
影响因子：
1.5
通讯作者：
A. Uchida
中科院分区：
文献类型：
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作者：
Takashi Yoshida;Y. Sako;A. Uchida
为了重新考虑毒素谱是否可以用作不同地理区域人群的标记，从大船渡湾（岩手县）、渥美湾（爱知县）、田边湾（和歌山县）、播磨滩（香川县）、浦之内湾对有毒甲藻塔玛亚历山大藻（Lebour）巴勒赫和链状亚历山大藻（Whedon et Kofoid）巴勒赫进行了克隆分离。采用形态分类学、免疫学和分子生物学技术鉴定出的麻痹性贝类中毒毒素，采用高效液相色谱-荧光法进行分析。除大船渡湾A. tamarense OF152外，所有分离物均主要含有N-磺基氨基甲酰毒素（C1+2）及不同量的衍生物，并观察到典型的从北到南毒性递减的趋势。在塔玛曲霉和链状曲霉中，毒素谱在一个地理区域内相当恒定，但在不同地理区域之间存在差异。 Harima-Nada 的 A. tamarense 的毒素谱在不同的开花年份中保存得很好。毒素谱显示，来自大船渡湾的塔玛曲霉、1988 年从 Harima-Nada 分离的塔玛曲霉和来自浦之内湾的链状曲霉是异质的。然而，在一个地理区域中仅观察到两组或三组具有不同毒素谱的分离株，这表明一些代表性分离株在给定区域表达该基因型。这些观察结果证实，毒素成分可以用作区分这些物种的不同地理种群的标记。
To reconsider whether toxin profile could be used as a marker for populations from different geographical areas, clonal isolates of the toxic dinoflagellates Alexandrium tamarense (Lebour) Balech and Alexandrium catenella (Whedon et Kofoid) Balech from Ofunato Bay (Iwate Prefecture), Atsumi Bay (Aichi Prefecture), Tanabe Bay (Wakayama Prefecture), Harima‐Nada (Kagawa Prefecture), Uranouchi Bay (Kochi Prefecture), Hiroshima Bay (Hiroshima Prefecture) and Yamakawa Bay (Kagoshima Prefecture), which were identified on the basis of morphotaxonomy, immunological and molecular biological techniques, were subjected to analysis of paralytic shellfish poisoning toxins by high performance liquid chromatography‐fluorometric method. All the isolates except A. tamarense OF152 from Ofunato Bay contained mainly N‐sulfocarbamoyl toxins (C1 +2) with various amounts of derivatives, and a typical north‐to‐south trend of decreasing toxicity was observed. In both A. tamarense and A. catenella, toxin profiles were rather constant within a geographical area and divergent among different geographical areas. The toxin profiles of A. tamarense from Harima‐Nada were well conserved among different bloom years. Toxin profile showed that isolates of A. tamarense from Ofunato Bay, A. tamarense from Harima‐Nada isolated in 1988 and A. catenella from Uranouchi Bay were heterogeneous. However, only two or three groups of isolates with different toxin profiles were observed in a geographical region, suggesting that several representative isolates express the genotype in a given region. These observations confirmed that toxin composition could be used as a marker to discriminate different geographical populations of these species.
Y.Sako 等人：“日本亚历山大藻（甲藻纲）核糖体 DNA 内转录间隔区和 5.8S 区域的限制性片段长度多态性。”
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绪方雄彦：TOXICON。
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