OPTICAL TRAPPING AND MANIPULATION OF SINGLE CELLS USING INFRARED-LASER BEAMS
OPTICAL TRAPPING AND MANIPULATION OF SINGLE CELLS USING INFRARED-LASER BEAMS
复制标题
DOI：
10.1038/330769a0
复制
发表时间：
1987-12-24
期刊：
影响因子：
64.8
通讯作者：
YAMANE, T
中科院分区：
文献类型：
--
作者：
ASHKIN, A;DZIEDZIC, JM;YAMANE, T
最近提出了使用光学陷阱来操纵生物粒子，并证实了用可见氩激光捕获细菌和病毒的初步观察结果1。我们在这里报告使用红外（IR）光，使大大改善激光陷阱与显着减少光学损伤的各种活细胞。利用红外光，我们观察到大肠杆菌在光阱中的繁殖，光阱的功率水平足以在高达100 µm s− 1的速度下进行操纵。酵母细胞的出芽繁殖也在能够在100 µm s−1的速度下操纵单个细胞和细胞团的IR阱中实现。无损伤的陷阱和操纵悬浮液的人的红细胞和细胞器位于个别活细胞的水绵也实现了，主要是由于减少吸收的血红蛋白和叶绿素的IR。许多类型的小型原生动物和操纵原生动物内的细胞器的陷阱也是可能的。光学技术的操纵能力被利用在实验中，显示从一个样品中分离单个细菌并将其引入另一个样品中。单个细菌细胞在空间中的光学定向也是使用一对激光束陷阱实现的。这些新的操纵技术使用红外线能够产生大的力量在无损伤的条件下，并提高了在微生物学中更广泛地使用光学操纵技术的前景。
Use of optical traps for the manipulation of biological particles was recently proposed, and initial observations of laser trapping of bacteria and viruses with visible argon-laser light were reported1. We report here the use of infrared (IR) light to make much improved laser traps with significantly less optical damage to a variety of living cells. Using IR light we have observed the reproduction ofEscherichia coliwithin optical traps at power levels sufficient to give manipulation at velocities up to ∼500 µm s−1Reproduction of yeast cells by budding was also achieved in IR traps capable of manipulating individual cells and clumps of cells at velocities of ∼100 µm s−1. Damage-free trapping and manipulation of suspensions of red blood cells of humans and of organelles located within individual living cells of spirogyra was also achieved, largely as a result of the reduced absorption of haemoglobin and chlorophyll in the IR. Trapping of many types of small protozoa and manipulation of organelles within protozoa is also possible. The manipulative capabilities of optical techniques were exploited in experiments showing separation of individual bacteria from one sample and their introduction into another sample. Optical orientation of individual bacterial cells in space was also achieved using a pair of laser-beam traps. These new manipulative techniques using IR light are capable of producing large forces under damage-free conditions and improve the prospects for wider use of optical manipulation techniques in microbiology.