Next-generation sequencing-based transcriptome analysis of Cryptolaemus montrouzieri under insecticide stress reveals resistance-relevant genes in ladybirds.
Next-generation sequencing-based transcriptome analysis of Cryptolaemus montrouzieri under insecticide stress reveals resistance-relevant genes in ladybirds.
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DOI：
10.1016/j.ygeno.2012.05.002
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发表时间：
2012-07
期刊：
影响因子：
4.4
通讯作者：
Yuhong Zhang;Ruixin Jiang;Hongsheng Wu;Ping Liu;Jiaqin Xie;Yunyu He;H. Pang
中科院分区：
文献类型：
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作者：
Yuhong Zhang;Ruixin Jiang;Hongsheng Wu;Ping Liu;Jiaqin Xie;Yunyu He;H. Pang
作为粉虱最有效的天敌，瓢虫Cryptolaemus montrouzieri Mulsant在害虫综合治理中发挥着重要作用。我们在此报告了对 C. montrouzieri 在杀虫剂胁迫下的概况分析，以更深入地了解瓢虫的杀虫剂抗性。对于转录组测序，产生了超过 2600 万个测序读数。这些读数被组装成 38,369 个非冗余转录本（平均大小 = 453 nt）。 23,248 个转录本带有基因描述注释。使用基于标签的 DGE（数字基因表达）系统，在杀虫剂应激组和对照组中对超过 570 万个标签进行了测序，并映射到 38,369 个转录本。我们在瓢虫转录组中获得了 993 个在杀虫剂胁迫下显着上调或下调的基因。这些结果有助于深入研究抗药性的分子机制，并增强我们目前对杀虫剂对天敌影响的认识。
As the most efficient natural enemy of mealybugs, the ladybird Cryptolaemus montrouzieri Mulsant plays an important role in integrated pest management. We report here a profiling analysis of C. montrouzieri under insecticide stress to gain a deeper view of insecticide resistance in ladybirds. For transcriptome sequencing, more than 26million sequencing reads were produced. These reads were assembled into 38,369 non-redundant transcripts (mean size=453 nt). 23,248 transcripts were annotated with their gene description. Using a tag-based DGE (Digital gene expression) system, over 5.7million tags were sequenced in both the insecticide stress group and the control group, and mapped to 38,369 transcripts. We obtained 993 genes that were significantly up- or down-regulated under insecticide stress in the ladybird transcriptome. These results can contribute to in-depth research into the molecular mechanisms of resistance and enhance our current understanding of the effects of insecticides on natural enemies.