Proteomic analysis of heterotrophy in Synechocystis sp PCC 6803
Proteomic analysis of heterotrophy in Synechocystis sp PCC 6803
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DOI：
10.1002/pmic.200500413
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发表时间：
2006-03-01
期刊：
影响因子：
3.4
通讯作者：
Mäenpää, P
中科院分区：
文献类型：
--
作者：
Kurian, D;Jansèn, T;Mäenpää, P
为了深入了解蓝藻的异养代谢，我们采用了蛋白质组学的方法来研究蓝藻的异养代谢。PCC 6803。采用双向凝胶电泳法对自养和光生异养合胞藻的可溶性蛋白质进行了分离，并用MALDI-MS或LC-MS/MS对其进行了鉴定。使用窄范围和微范围IPG条制作的2-DE凝胶可以对900多个斑点进行定量比较。在鉴定出的67个丰富的蛋白质点中，13个点在异养状态下增加，9个点减少，代表了所有主要的折叠变化。蛋白质组的改变和所选择的酶的活性水平表明，中央碳代谢的变化是对营养变化的响应。异养条件下，光饱和光合作用速率以及Rubisco和CO2聚集机制蛋白的表达水平显著降低，表明光合作用机制的下调。碳利用途径相关蛋白表达水平的变化是指异养条件下糖酵解增强、磷酸戊糖氧化途径和三羧酸循环的变化。蛋白质组学证据还表明，在异养生长过程中，组氨酸和丝氨酸等氨基酸的生物合成增加。
To provide an insight into the heterotrophic metabolism of cyanobacteria, a proteomic approach has been employed with the model organism Synechocystis sp. PCC 6803. The soluble proteins from Synechocystis grown under photoautotrophic and light-activated heterotrophic conditions were separated by 2-DE and identified by MALDI-MS or LC-MS/MS analysis. 2-DE gels made using narrow- and micro-range IPG strips allowed quantitative comparison of more than 900 spots. Out of 67 abundant protein spots identified, 13 spots were increased and 9 decreased under heterotrophy, representing all the major fold changes. Proteomic alterations and activity levels of selected enzymes indicate a shift in the central carbon metabolism in response to trophic change. The significant reduction in light-saturated rate of photosynthesis as well as in the expression levels of rubisco and CO2-concentrating mechanism proteins under heterotrophy indicates the down-regulation of the photosynthetic machinery. Alterations in the expression level of proteins involved in carbon utilization pathways refer to enhanced glycolysis, oxidative pentose phosphate pathway as well as tricarboxylic acid cycle under heterotrophy. Proteomic evidences also suggest an enhanced biosynthesis of amino acids such as histidine and serine during heterotrophic growth.