Identification of a novel P450 gene belonging to the CYP4 family in the clam Ruditapes philippinarum, and analysis of basal- and benzo(a)pyrene-induced mRNA expression levels in selected tissues.
Identification of a novel P450 gene belonging to the CYP4 family in the clam Ruditapes philippinarum, and analysis of basal- and benzo(a)pyrene-induced mRNA expression levels in selected tissues.
复制标题
DOI：
10.1016/j.etap.2011.08.005
复制
发表时间：
2011-11
影响因子：
4.3
通讯作者：
L. Pan;Na Liu;Chaoqun Xu;Jingjing Miao
中科院分区：
文献类型：
--
作者：
L. Pan;Na Liu;Chaoqun Xu;Jingjing Miao
编码 CYP4 蛋白的新型全长 cDNA 最初是从菲律宾蛤仔中克隆出来的。该核苷酸序列包含编码442个氨基酸的开放阅读框，推导的氨基酸序列与其他物种CYP4s具有42.6-49.1%的同一性。系统发育分析表明蛤 CYP4 聚集在 CYP4 分支内。蛤的CYP4 mRNA在鳃、消化腺、内收肌和外套膜中检测到表达，与其他组织相比，消化腺中的转录水平最高。实时定量RT-PCR分析显示，苯并(a)芘(BaP)处理后菲律宾蛤鳃中CYP4 mRNA表达没有显着变化，而0.2ppb (μgL−1) BaP显着诱导蛤消化腺中CYP4 mRNA表达(p<0.05)。结果表明，蛤的 CYP4 可能作为海洋环境 PAH 污染的有用生物标志物。
A novel full-length cDNA encoding a CYP4 protein was initially cloned from the clam, Ruditapes philippinarum. The nucleotide sequence contained an open reading frame coding for 442 amino acids and the deduced amino acid sequence showed 42.6–49.1% identity with other species CYP4s. The phylogenetic analysis demonstrated that the clam CYP4 was clustered within the CYP4s branch. The clam CYP4 mRNA expression was detected in gill, digestive gland, adductor muscle and mantle, and highest transcription level was observed in digestive gland compared to other tissues. Quantitative real-time RT-PCR analysis revealed that there was no notable change in CYP4 mRNA expression in gill of R. philippinarum exposure to benzo(a)pyrene (BaP), while the mRNA expression was induced significantly in the digestive gland of the clam by 0.2ppb (μgL−1) BaP (p<0.05). The results suggest that CYP4 of the clam may serve as a useful biomarker of marine environmental PAH pollution.