Screening of the Antarctic marine sponges (Porifera) as a source of bioactive compounds
Screening of the Antarctic marine sponges (Porifera) as a source of bioactive compounds
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DOI：
10.1007/s00300-015-1835-4
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发表时间：
2016-05-01
期刊：
影响因子：
1.7
通讯作者：
Sepcic, Kristina
中科院分区：
文献类型：
--
作者：
Berne, Sabina;Kalauz, Martina;Sepcic, Kristina
海绵（多孔动物）目前是最丰富的天然产物来源之一，占海洋来源的生物活性化合物的近一半。然而，迄今为止，人们对极地海绵的药理潜力知之甚少。在这项工作中，我们报告的筛选乙醇提取物从24个南极海绵不同的生物活性。测试了提取物对正常细胞系和转化细胞系、红细胞和藻类的细胞毒性作用，对选定的生理学重要酶（乙酰胆碱酯酶、丁酰胆碱酯酶和α-淀粉酶）的活性的调节，以及对致病和生态相关细菌和真菌生长的抑制。一个提取物从Tedania（Tedaniopsis）oxeata选择性细胞毒性对癌细胞系，并显示所有测试的生态相关的和潜在的致病性真菌菌株的生长抑制。海绵提取物对胆碱酯酶活性有抑制作用，而海绵提取物对α-淀粉酶活性有抑制作用。几种海绵提取物对不同来源的多重耐药病原菌的生长有抑制作用，包括超广谱β-内酰胺酶和碳青霉烯类耐药菌株，而来自K. variolosa和Myxilla（Myxilla）mollis对人耐甲氧西林金黄色葡萄球菌菌株有活性。我们的结论是，南极海绵是一个有价值的来源，具有药理学潜力的生物活性化合物。
Sponges (Porifera) currently represent one of the richest sources of natural products and account for almost half of the pharmacologically active compounds of marine origin. However, to date very little is known about the pharmacological potential of the sponges from polar regions. In this work we report on screening of ethanolic extracts from 24 Antarctic marine sponges for different biological activities. The extracts were tested for cytotoxic effects against normal and transformed cell lines, red blood cells, and algae, for modulation of the activities of selected physiologically important enzymes (acetylcholinesterase, butyrylcholinesterase, and alpha-amylase), and for inhibition of growth of pathogenic and ecologically relevant bacteria and fungi. An extract from Tedania (Tedaniopsis) oxeata was selectively cytotoxic against the cancer cell lines and showed growth inhibition of all of the tested ecologically relevant and potentially pathogenic fungal isolates. The sponge extracts from Isodictya erinacea and Kirkpatrickia variolosa inhibited the activities of the cholinesterase enzymes, while the sponge extracts from Isodictya lankesteri and Inflatella belli reduced the activity of alpha-amylase. Several sponge extracts inhibited the growth of multiresistant pathogenic bacterial isolates of different origins, including extended-spectrum beta-lactamase and carbapenem-resistant strains, while sponge extracts from K. variolosa and Myxilla (Myxilla) mollis were active against a human methicillin-resistant Staphylococcus aureus strain. We conclude that Antarctic marine sponges represent a valuable source of biologically active compounds with pharmacological potential.