Human antibody response to Campylobacter jejuni flagellin protein and a synthetic N-terminal flagellin peptide.
Human antibody response to Campylobacter jejuni flagellin protein and a synthetic N-terminal flagellin peptide.
复制标题
对空肠弯曲杆菌鞭毛蛋白和合成 N 末端鞭毛蛋白肽的人类抗体反应。
DOI：
10.1128/jcm.27.10.2195-2198.1989
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发表时间：
1989
影响因子：
9.4
通讯作者：
Yang,XH
中科院分区：
文献类型：
--
作者：
Nachamkin,I;Yang,XH
我们测量了同种型特异性的人抗体针对空肠弯曲菌天然鞭毛蛋白和合成肽来自N-末端氨基酸序列的蛋白质，通过使用微量稀释酶联免疫吸附试验（ELISA）。C.胃肠道感染患者血清标本。空肠（n = 20）和对照样品（来自正常受试者的数量= 20;来自除弯曲杆菌外的腹泻患者的数量= 20）在该测定中进行测试。弯曲杆菌感染患者的血清标本显示，同种型特异性抗鞭毛蛋白抗体滴度比对照样品显着更高。免疫球蛋白G（IgG）抗体检测的特异性（70%）低于伊加或IgM抗体在感染患者（95%）的检测。在急性期血清标本中，任何同种型检测的灵敏度范围为64 - 100%，在恢复期血清标本中为85 - 95%。以源自鞭毛蛋白的N末端合成肽作为抗原的ELISA对于检测弯曲杆菌感染不敏感（60%），但特异性非常高（97.5%）。结论：血清伊加C.空肠鞭毛蛋白可能是一种有用的感染标志物。虽然N-末端合成肽在少数感染患者中具有抗原性，并且在ELISA中显示出良好的特异性，但需要鉴定用于检测感染的具有更好灵敏度的额外氨基酸序列。
We measured isotype-specific human antibodies directed against Campylobacter jejuni native flagellin and a synthetic peptide derived from the N-terminal amino acid sequence of the protein by using a microdilution enzyme-linked immunosorbent assay (ELISA). Serum samples from patients with gastrointestinal infection caused by C. jejuni (n = 20) and control samples (number from normal subjects = 20; number from patients with diarrhea other than campylobacter = 20) were tested in this assay. Serum specimens from patients with campylobacter infection showed statistically significant higher isotype-specific antiflagellin antibody titers than control samples did. Detection of immunoglobulin G (IgG) antibodies was less specific (70%) than detection of either IgA or IgM antibodies in infected patients (95%). The sensitivity of testing for any of the isotypes ranged from 64 to 100% in acute-phase serum specimens and 85 to 95% in convalescent-phase serum specimens. An ELISA with an N-terminal synthetic peptide derived from the flagellin protein as antigen was not sensitive (60%) for detecting campylobacter infection but was very specific (97.5%). In conclusion, detection of serum IgA or IgM against C. jejuni flagellin may be a useful marker of infection. Although the N-terminal synthetic peptide was antigenic in a few patients with infection and showed good specificity in the ELISA, additional amino acid sequences with better sensitivity for detecting infection need to be identified.