The Mouse Model of Infection with Citrobacter rodentium.
The Mouse Model of Infection with Citrobacter rodentium.
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DOI：
10.1002/cpim.34
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发表时间：
2017-11-01
影响因子：
--
通讯作者：
Belkaid Y
中科院分区：
文献类型：
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作者：
Bouladoux N;Harrison OJ;Belkaid Y
啮齿类柠檬酸杆菌是一种小鼠粘膜病原体，用作阐明两种临床上重要的人类胃肠道病原体（肠致病性大肠杆菌（EPEC）和肠出血性大肠杆菌）感染的分子和细胞发病机制的模型。大肠杆菌（EHEC）。C.啮齿动物感染提供了一个很好的模型来研究肠道中宿主-病原体相互作用的不同方面，包括细菌诱导的结肠炎期间的肠道炎症反应、粘膜愈合和上皮修复、粘膜免疫反应的诱导以及肠道微生物群在介导对肠道病原体定殖的抗性中的作用。在这个单元中，我们提供了详细的协议，以培养这种细菌，感染小鼠胃内接种，测量粪便和器官中的细菌负荷，并监测这种感染引起的肠道病理学。附加的实验方案描述了建立冻存物、建立C.啮齿动物，进行离体器官培养，从大肠分离免疫细胞，并通过流式细胞术测量免疫应答。
Citrobacter rodentium is a murine mucosal pathogen used as a model to elucidate the molecular and cellular pathogenesis of infection with two clinically important human gastrointestinal pathogens, enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC). C. rodentium infection provides an excellent model to study different aspects of host-pathogen interaction in the gut, including intestinal inflammatory responses during bacteria-induced colitis, mucosal healing and epithelial repair, the induction of mucosal immune responses and the role of the intestinal microbiota in mediating resistance to colonization by enteric pathogens. In this unit, we provide detailed protocols to grow this bacterium, infect mice by intragastric inoculation, measure bacterial loads in feces and organs and monitor the intestinal pathology induced by this infection. Additional protocols describe steps needed to create frozen stocks, establish a growth curve of C. rodentium, perform ex vivo organ cultures, isolate immune cells from the large intestine and measure immune response by flow cytometry.