In vivo 13C magnetic resonance spectroscopy of a human brain tumor after application of 13C-1-enriched glucose
In vivo 13C magnetic resonance spectroscopy of a human brain tumor after application of 13C-1-enriched glucose
复制标题
DOI：
10.1016/j.mri.2010.03.006
复制
发表时间：
2010-06-01
影响因子：
2.5
通讯作者：
Heerschap, Arend
中科院分区：
文献类型：
--
作者：
Wijnen, Jannie P.;Van der Graaf, Marinette;Heerschap, Arend
目的：作为一种独特的无创评估代谢通量的工具，C-13磁共振波谱（MRS）可以帮助表征和了解人类肿瘤的恶性程度。然而，它的低灵敏度阻碍了在患者中的应用。本研究的目的是证明，在正常血糖状态下，通过敏感性优化的局部C-13 MRS和静脉输注[1-C-13]葡萄糖，可以评估葡萄糖在人胶质瘤组织体内向其代谢产物的动态转化。材料和方法：在3t下使用宽带单射频通道和插入H-1体积线圈的正交C-13表面线圈进行测量。应用H-1/C-13极化转移序列，修改为局部采集，交替在两个（50 ml）体素中，一个包含肿瘤，另一个包含正常脑组织。结果：[1-C-13]葡萄糖输注约20 min后，肿瘤体素MR谱中葡萄糖代谢产物的多个共振中出现了[3-C-13]乳酸信号。[3-C-13]乳酸的共振在对侧组织的磁共振光谱中不存在。此外，肿瘤区域的[1-C-13]葡萄糖信号强度比正常组织高50%左右，可能是由于血脑屏障缺陷导致细胞外空间葡萄糖增多。三羧酸（TCA）循环产生的代谢物的信号强度在肿瘤中比在对侧区域低，尽管同位素体信号的比率是相当的。结论：采用改进的C-13 MRS方法，可以无创地监测脑肿瘤中葡萄糖的摄取及其转化为乳酸等代谢物的过程。这为评估人类肿瘤组织的代谢活动开辟了道路。(C) 2010爱思唯尔公司版权所有。
Objectives: As a unique tool to assess metabolic fluxes noninvasively, C-13 magnetic resonance spectroscopy (MRS) could help to characterize and understand malignancy in human tumors. However, its low sensitivity has hampered applications in patients. The aim of this study was to demonstrate that with sensitivity-optimized localized C-13 MRS and intravenous infusion of [1-C-13]glucose under euglycemia, it is possible to assess the dynamic conversion of glucose into its metabolic products in vivo in human glioma tissue.Materials and Methods: Measurements were done at 3 T with a broadband single RF channel and a quadrature C-13 surface coil inserted in a H-1 volume coil. A H-1/C-13 polarization transfer sequence was applied, modified for localized acquisition, alternatively in two (50 ml) voxels, one encompassing the tumor and the other normal brain tissue.Results: After about 20 min of[1-C-13]glucose infusion, a [3-C-13]lactate signal appeared among several resonances of metabolic products of glucose in MR spectra of the tumor voxel. The resonance of [3-C-13]lactate was absent in MR spectra from contralateral tissue. In addition, the intensity of [1-C-13]glucose signals in the tumor area was about 50% higher than that in normal tissue, likely reflecting more glucose in extracellular space due to a defective blood brain barrier. The signal intensity for metabolites produced in or via the tricarboxylic acid (TCA) cycle was lower in the tumor than in the contralateral area, albeit that the ratios of isotopomer signals were comparable.Conclusion: With an improved C-13 MRS approach, the uptake of glucose and its conversion into metabolites such as lactate can be monitored noninvasively in vivo in human brain tumors. This opens the way to assessing metabolic activity in human tumor tissue. (C) 2010 Elsevier Inc. All rights reserved.