Biomaterials Science
Biomaterials Science
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DOI：
10.1039/2047-4849/2013
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通讯作者：
Ziyuan Song;Yee Ming Khaw;Lazaro A Pacheco;Kuan-Ying Tseng;Zhengzhong Tan;Kaimin Cai;Ettigounder Ponnusamy;Jianjun Cheng;Makoto Inoue
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作者：
Ziyuan Song;Yee Ming Khaw;Lazaro A Pacheco;Kuan-Ying Tseng;Zhengzhong Tan;Kaimin Cai;Ettigounder Ponnusamy;Jianjun Cheng;Makoto Inoue
在过去的几十年里，非热等离子体作为各种生物医学应用的相关工具得到了广泛的研究，从组织净化到再生，从皮肤治疗到肿瘤治疗。这种高通用性是由于在等离子体处理过程中可以产生不同种类和数量的活性氧和氮，并与生物靶标接触。最近的一些研究报告说，具有生成水凝胶能力的生物聚合物溶液在用等离子体处理时，可以增强反应性物质的生成并影响其稳定性，从而成为间接处理生物靶点的理想介质。等离子体处理对水溶液中生物聚合物结构的直接影响，以及增强RONS生成的化学机制尚不完全清楚。在这项研究中，我们的目标是通过调查来填补这一空白，一方面，藻酸盐溶液中等离子体处理引起的改变的性质和程度，另一方面，利用这些信息来解释作为处理结果的反应性物质产生增强的机制。我们使用的方法是双重的：(i)研究等离子体处理对海藻酸盐溶液的影响，通过尺寸排除色谱，流变学和扫描电子显微镜；（ii）研究一个分子模型（葡萄糖酸盐）共享其化学结构，通过色谱联用质谱和分子动力学模拟。我们的研究结果指出了生物聚合物化学在直接等离子体处理中的积极作用。短寿命的活性物质，如OH自由基和O原子，可以改变聚合物的结构，从而影响其官能团并引起部分断裂。其中一些化学阳离子，如有机过氧化物的生成，可能会导致二次生成长寿命的活性物质，如过氧化氢和亚硝酸盐离子。鉴于使用生物相容性水凝胶作为靶向治疗的储存和递送反应性物质的载体，这是相关的。
In the last decades, non-thermal plasma has been extensively investigated as a relevant tool for various biomedical applications, ranging from tissue decontamination to regeneration and from skin treatment to tumor therapies. This high versatility is due to the di ff erent kinds and amount of reactive oxygen and nitrogen species that can be generated during a plasma treatment and put in contact with the biological target. Some recent studies report that solutions of biopolymers with the ability to generate hydrogels, when treated with plasma, can enhance the generation of reactive species and in fl uence their stability, resulting thus in the ideal media for indirect treatments of biological targets. The direct e ff ects of the plasma treatment on the structure of biopolymers in water solution, as well as the chemical mechanisms responsible for the enhanced generation of RONS, are not yet fully understood. In this study, we aim at fi lling this gap by investigating, on the one hand, the nature and extent of the modi fi cations induced by plasma treatment in alginate solutions, and, on the other hand, at using this information to explain the mechanisms responsible for the enhanced generation of reactive species as a consequence of the treatment. The approach we use is twofold: (i) investigating the e ff ects of plasma treatment on alginate solutions, by size exclusion chromatography, rheology and scanning electron microscopy and (ii) study of a molecular model (glucuro-nate) sharing its chemical structure, by chromatography coupled with mass spectrometry and by molecular dynamics simulations. Our results point out the active role of the biopolymer chemistry during direct plasma treatment. Short-lived reactive species, such as OH radicals and O atoms, can modify the polymer structure, a ff ecting its functional groups and causing partial fragmentation. Some of these chemical modi fi cations, like the generation of organic peroxide, are likely responsible for the secondary generation of long-lived reactive species such as hydrogen peroxide and nitrite ions. This is relevant in view of using biocompatible hydrogels as vehicles for storage and delivery reactive species for targeted therapies.