APEX1 Expression as a Potential Diagnostic Biomarker of Clear Cell Renal Cell Carcinoma and Hepatobiliary Carcinomas
APEX1 Expression as a Potential Diagnostic Biomarker of Clear Cell Renal Cell Carcinoma and Hepatobiliary Carcinomas
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DOI：
10.3390/jcm8081151
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发表时间：
2019-08-01
影响因子：
3.9
通讯作者：
Kim, Kyung-Hee
中科院分区：
文献类型：
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作者：
Kim, Ji-Myung;Yeo, Min-Kyung;Kim, Kyung-Hee
脱嘌呤/脱嘧啶核酸内切酶1/氧化还原效应因子1（APEX 1）在DNA修复、调节多种转录活性和细胞对氧化还原活性的反应中起关键作用。本研究旨在检测血清APEX 1（s-APEX 1）表达作为透明细胞肾细胞癌（ccRCC）、肝细胞癌（HCC）以及近端和远端胆管癌（CC）的可能筛选生物标志物。从39例健康对照病例、32例B型肝炎病毒DNA（HBV DNA（+））>= 58拷贝/mL的患者、40例ccRCC病例、59例HCC病例和46例CC病例中共收集了216份冷冻血清样本。采用酶联免疫吸附试验检测血清中s-APEX 1的浓度。在106例ccRCC、131例HCC和32例肝内CC病例中，还通过免疫组化染色研究了APEX 1表达与临床病理特征的关系。HCC、CC、ccRCC、健康对照和HBV DNA（+）组的中位s-APEX 1浓度分别为0.294、0.710、0.474、0.038和2.384 ng/mL（p < 0.001）。单因素和多因素分析显示，在HCC和CC病例中，细胞质APEX 1表达增加导致无病生存期缩短。我们认为s-APEX 1水平可能是ccRCC，HCC和CC的潜在诊断生物标志物。此外，癌细胞中的细胞质APEX 1表达可用于预测HCC或CC患者的复发。
Apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APEX1) has been known to play key roles in DNA repair, the regulation of diverse transcriptional activity, and cellular responses to redox activity. This study aimed to examine serum APEX1 (s-APEX1) expression as a possible screening biomarker for clear cell renal cell carcinoma (ccRCC), hepatocellular carcinoma (HCC), and proximal and distal cholangiocarcinoma (CC). A total of 216 frozen serum samples were collected from 39 healthy control cases, 32 patients with >= 58 copies/mL of hepatitis B viral DNA (HBV DNA (+)), 40 ccRCC cases, 59 HCC cases, and 46 CC cases. The serum samples were examined for s-APEX1 concentration by enzyme-linked immunosorbent assay. The association of APEX1 expression with clinicopathological characteristics was also studied by immunohistochemical staining in 106 ccRCC, 131 HCC, and 32 intrahepatic CC cases. The median s-APEX1 concentrations of the HCC, CC, ccRCC, healthy control, and HBV DNA (+) groups were 0.294, 0.710, 0.474, 0.038, and 2.384 ng/mL, respectively (p < 0.001). Univariate and multivariate analyses revealed that increased cytoplasmic APEX1 expression led to a shorter disease-free survival period in HCC and CC cases. We suggest that the s-APEX1 level could be a potential diagnostic biomarker of ccRCC, HCC, and CC. Additionally, cytoplasmic APEX1 expression in cancer cells could be used to predict relapses in patients with HCC or CC.