A barrier against reactive oxygen species: chitosan/acellular dermal matrix scaffold enhances stem cell retention and improves cutaneous wound healing
A barrier against reactive oxygen species: chitosan/acellular dermal matrix scaffold enhances stem cell retention and improves cutaneous wound healing
复制标题
活性氧屏障：壳聚糖/脱细胞真皮基质支架可增强干细胞保留并改善皮肤伤口愈合
DOI：
10.1186/s13287-020-01901-6
复制
发表时间：
2020-09-07
影响因子：
7.5
通讯作者：
Deng, Xiaoyuan
中科院分区：
文献类型：
--
作者：
Lin, Wei;Qi, Xiaoyang;Deng, Xiaoyuan
背景干细胞疗法由于其自我更新、分化为各种细胞类型的能力以及良好的旁分泌功能而为治疗各种损伤和疾病提供了新的解决方案，因此受到了极大的关注。然而，移植干细胞的低保留率仍然限制了其临床应用，如伤口愈合，因为在炎症反应过程中诱导的苛刻的微环境富含活性氧（ROS）。该干细胞递送系统充当屏障，有效地清除一定量的ROS并保护间充质干细胞（MSC）免受氧化应激。它显著调节MSC中的细胞内ROS水平并减少ROS诱导的细胞死亡。最重要的是，这样的骨髓间充质干细胞输送系统显着提高在体内移植的干细胞滞留，促进血管生长，并加速伤口愈合.ConclusionsThis新的输送系统，克服了传统的平原胶原蛋白为基础的输送系统缺乏ROS-环境响应机制的局限性，表现出巨大的潜力，在干细胞治疗伤口愈合。
BackgroundStem cell therapies have gained great attention for providing novel solutions for treatment of various injuries and diseases due to stem cells' self-renewal, ability to differentiate into various cell types, and favorite paracrine function. Nevertheless, the low retention of transplanted stem cell still limits their clinical applications such as in wound healing in view of an induced harsh microenvironment rich in reactive oxygen species (ROS) during inflammatory reactions.MethodsHerein, a novel chitosan/acellular dermal matrix (CHS/ADM) stem cell delivery system is developed, which is of great ROS scavenging activity and significantly attenuates inflammatory response.ResultUnder ROS microenvironment, this stem cell delivery system acts as a barrier, effectively scavenging an amount of ROS and protecting mesenchymal stem cells (MSCs) from the oxidative stress. It notably regulates intracellular ROS level in MSCs and reduces ROS-induced cellular death. Most importantly, such MSCs delivery system significantly enhances in vivo transplanted stem cell retention, promotes the vessel growth, and accelerates wound healing.ConclusionsThis novel delivery system, which overcomes the limitations of conventional plain collagen-based delivery system in lacking of ROS-environmental responsive mechanisms, demonstrates a great potential use in stem cell therapies in wound healing.