In vitro antibacterial activity of oxide and non-oxide bioceramics for arthroplastic devices: I. In situ time-lapse Raman spectroscopy
In vitro antibacterial activity of oxide and non-oxide bioceramics for arthroplastic devices: I. In situ time-lapse Raman spectroscopy
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DOI：
10.1039/c8an00233a
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发表时间：
2018-08-07
期刊：
影响因子：
4.2
通讯作者：
Bal, Sonny B.
中科院分区：
文献类型：
--
作者：
Pezzotti, Giuseppe;Bock, Ryan M.;Bal, Sonny B.
在接下来的二十年中，预计对关节成形器械的强劲人口需求加上抗生素疗效的下降将导致假体周围关节感染（PJIs）数量的指数增加。因此，需要先进的策略来改善植入物周围的局部免疫反应，并抑制细菌粘附和生物膜形成的致病事件。使用自主对抗感染的生物材料是改善骨科结局的一种方法。使用传统的分子生物学表征方法和先进的拉曼光谱，本研究探讨了两种生物陶瓷材料的抑菌反应，通常采用作为假体植入物：氧化锆增韧氧化铝（ZTA）和氮化硅（Si3N4）。与ZTA不同，发现非氧化物Si3N4具有固有的抗感染表面化学，其以响应的方式对抗细菌负载。阐明了其行为的机理细节。非氧化物生物陶瓷似乎是有前途的，但他们的全面发展需要一个过渡的方法，将基本的生物化学概念与临床结果相结合。
Over the next two decades, a strong demographic demand for arthroplastic devices coupled with a decreased efficacy of antibiotics has been predicted to result in an exponential increase in the number of periprosthetic joint infections (PJIs). Advanced strategies are therefore required to improve the local peri-implant immune response and curb the pathogenic events of bacterial adhesion and biofilm formation. The use of biomaterials that autonomously counter infections is one approach to improve orthopedic outcomes. Using conventional molecular biology characterization methods and advanced Raman spectroscopy, this study examined the bacteriostatic response of two bioceramic materials commonly employed as prosthetic implants: zirconia-toughened alumina (ZTA) and silicon nitride (Si3N4). Unlike the ZTA, it was found that non-oxide Si3N4 possesses an inherently anti-infective surface chemistry, which acts in a responsive way against bacterial loading. The mechanistic details of its behavior are elucidated. Non-oxide bioceramics appear to be promising, but their full development requires a transitional approach that integrates the fundamental biochemical concepts with clinical outcomes.