A thermodynamic investigation into the binding properties of DNA functionalized gold nanoparticle probes and molecular fluorophore probes
A thermodynamic investigation into the binding properties of DNA functionalized gold nanoparticle probes and molecular fluorophore probes
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DOI：
10.1021/ja052255o
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发表时间：
2005-09-21
影响因子：
15
通讯作者：
Mirkin, CA
中科院分区：
文献类型：
--
作者：
Lytton-Jean, AKR;Mirkin, CA
我们首次对DNA功能化的金纳米颗粒探针的寡核苷酸结合热力学进行了定量分析，并在序列对序列的基础上将我们的发现与分子荧光团探针进行了比较。在适当的设计下，在相同的条件下，纳米颗粒探针与分子荧光探针的结合显著增加。这一点意义重大，因为探针结合强度直接影响检测灵敏度极限。
We report the first quantitative analysis of the oligonucleotide binding thermodynamics for DNA functionalized gold nanoparticle probes and compare our findings to molecular fluorophore probes on a sequence-for-sequence basis. With proper design, nanoparticle probes show significantly increased binding over molecular fluorophore probes under identical conditions. This is significant because probe binding strength directly influences detection sensitivity limits.