Transneuronal circuit analysis with pseudorabies viruses.
Transneuronal circuit analysis with pseudorabies viruses.
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DOI：
10.1002/0471142301.ns0105s68
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发表时间：
2014-07-01
影响因子：
--
通讯作者：
Enquist, Lynn W
中科院分区：
文献类型：
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作者：
Card, J Patrick;Enquist, Lynn W
我们理解神经系统功能的能力依赖于定义其组成神经元的连接。定义神经网络内部联系的方法的开发一直是神经科学中的一个成长性行业。目前，嗜神经病毒的跨神经元传播是定义神经网络中突触连接的最佳手段。这种方法利用病毒入侵神经元、复制和传播的能力，通过使神经元之间能够通信的亲密突触连接进行传播。自20世纪70年代首次引入该方法以来，它受益于对病毒生命周期、病毒基因组功能以及操纵病毒基因组以支持病毒定向传播和转基因表达的能力的了解。在这一单元中，我们回顾了病毒追踪技术的这些进展，以及它们可能被应用于神经网络的功能解剖的方式。
Our ability to understand the function of the nervous system is dependent upon defining the connections of its constituent neurons. Development of methods to define connections within neural networks has always been a growth industry in the neurosciences. Transneuronal spread of neurotropic viruses currently represents the best means of defining synaptic connections within neural networks. The method exploits the ability of viruses to invade neurons, replicate, and spread through the intimate synaptic connections that enable communication among neurons. Since the method was first introduced in the 1970s, it has benefited from an increased understanding of the virus life cycle, the function of viral genome, and the ability to manipulate the viral genome in support of directional spread of virus and the expression of transgenes. In this unit, we review these advances in viral tracing technology and the way in which they may be applied for functional dissection of neural networks.