Zebrafish as a model for vertebrate hematopoiesis
Zebrafish as a model for vertebrate hematopoiesis
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DOI：
10.1016/j.coph.2010.05.004
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发表时间：
2010-10-01
影响因子：
4
通讯作者：
Lieschke, Graham J.
中科院分区：
文献类型：
--
作者：
Ellett, Felix;Lieschke, Graham J.
斑马鱼（Danio rerio）是一种模式生物，在许多生物学研究领域做出了有益的贡献。斑马鱼被证明特别适合研究早期发育。斑马鱼胚胎的透明性和离体发育意味着早期胚胎学可以很容易地可视化，特别是使用表达荧光团标记感兴趣组织的转基因菌株。高繁殖力和耐受密集诱变使其成为正向遗传筛选和创建诱变文库的实用模型，从中可以回收稳定的突变等位基因。通过显微注射mRNA（过表达）或反义吗啉代寡核苷酸（敲低）的瞬时遗传操作提供了用于在功能上评估遗传调控途径而不需要扩展育种策略的方便方法。该模型的效用的一个突出的例子是其应用于造血的最早阶段的建模。斑马鱼发育性造血与哺乳动物造血系统的发育密切相关，并受保守的分子途径调控。这篇综述强调了最近的关键研究，使用这个模型提供了深入了解脊椎动物造血发育和先天免疫。
The zebrafish (Danio rerio) is a model organism making useful contributions in many areas of biological research. Zebrafish have proven particularly suitable for studying early development. The transparency and ex vivo development of zebrafish embryos means that early embryology can be easily visualized, especially using transgenic strains expressing fluorophores marking tissues of interest. High fecundity and tolerance of dense mutagenesis have made it a practical model for forward genetic screening and creation of mutagenized libraries from which stable mutant alleles can be recovered. Transient genetic manipulation by microinjection of mRNA (overexpression) or antisense morpholino oligonucleotides (knockdown) provide convenient methods for functionally assessing genetic regulatory pathways without the need for extended breeding strategies. A standout example of the utility of this model has been its application to modeling of the earliest stages of hematopoiesis. Zebrafish developmental hematopoiesis shows close correspondence to the development of the mammalian hematopoietic system and is regulated by conserved molecular pathways. This review highlights key recent studies that have used this model to provide insights into vertebrate hematopoietic development and innate immunity.