Plant-Derived Exosomal MicroRNAs Shape the Gut Microbiota
Plant-Derived Exosomal MicroRNAs Shape the Gut Microbiota
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DOI：
10.1016/j.chom.2018.10.001
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发表时间：
2018-11-14
影响因子：
30.3
通讯作者：
Zhang, Huang-Ge
中科院分区：
文献类型：
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作者：
Teng, Yun;Ren, Yi;Zhang, Huang-Ge
饮食干预可以改变肠道微生物群，以预防和治疗各种疾病。然而，食品调节共生体的机制仍然很大程度上未知。我们证明植物源性外泌体样纳米粒子 (ELN) 被肠道微生物群吸收，并含有改变微生物组组成和宿主生理学的 RNA。生姜 ELN (GELN) 优先被乳杆菌科以 GELN 脂质依赖性方式摄取，并且含有针对鼠李糖乳杆菌 (LGG) 中各种基因的 microRNA。其中，GELN mdo-miR7267-3p 介导的 LGG 单加氧酶 ycnE 的靶向产量增加了吲哚-3-甲醛 (I3A)。 GELN-RNA 或 I3A（芳烃受体的配体）足以诱导 IL-22 的产生，而 IL-22 与屏障功能的改善有关。 GELN-RNA 的这些功能可以通过 IL-22 依赖性机制改善小鼠结肠炎。这些发现揭示了植物产品及其对微生物组的影响如何用于针对特定宿主过程以减轻疾病。
The gut microbiota can be altered by dietary interventions to prevent and treat various diseases. However, the mechanisms by which food products modulate commensals remain largely unknown. We demonstrate that plant-derived exosome-like nanoparticles (ELNs) are taken up by the gut microbiota and contain RNAs that alter microbiome composition and host physiology. Ginger ELNs (GELNs) are preferentially taken up by Lactobacillaceae in a GELN lipid-dependent manner and contain microRNAs that target various genes in Lactobacillus rhamnosus (LGG). Among these, GELN mdo-miR7267-3p-mediated targeting of the LGG monooxygenase ycnE yields increased indole-3-carboxaldehyde (I3A). GELN-RNAs or I3A, a ligand for aryl hydrocarbon receptor, are sufficient to induce production of IL-22, which is linked to barrier function improvement. These functions of GELN-RNAs can ameliorate mouse colitis via IL-22-dependent mechanisms. These findings reveal how plant products and their effects on the microbiome may be used to target specific host processes to alleviate disease.