Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos.
Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos.
复制标题
小鼠植入前胚胎中线性和环状 RNA 的单细胞 RNA-seq 转录组分析。
DOI：
10.1186/s13059-015-0706-1
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发表时间：
2015-07-23
期刊：
影响因子：
12.3
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Fan X;Zhang X;Wu X;Guo H;Hu Y;Tang F;Huang Y
环状RNA（CircRNA）是一类新的非多聚腺苷酸化的非编码RNA，在许多生物学过程中发挥着重要作用。在这里，我们开发了一种单细胞通用poly（A）-独立的RNA测序（SUPeR-seq）方法，对来自单个细胞的多聚腺苷酸化和非多聚腺苷酸化RNA进行测序。该方法具有稳健的灵敏度、精密度和准确度。我们在小鼠植入前胚胎中发现了2891个circRNA和913个新的线性转录本，并进一步分析了circRNA沿着发育的丰度、富集基因的功能以及circRNA的序列特征。我们的工作是破译哺乳动物早期胚胎发育过程中circRNA调控机制的关键。本文的在线版本（doi：10.1186/s13059-015-0706-1）包含补充材料，可供授权用户使用。
Circular RNAs (circRNAs) are a new class of non-polyadenylated non-coding RNAs that may play important roles in many biological processes. Here we develop a single-cell universal poly(A)-independent RNA sequencing (SUPeR-seq) method to sequence both polyadenylated and non-polyadenylated RNAs from individual cells. This method exhibits robust sensitivity, precision and accuracy. We discover 2891 circRNAs and 913 novel linear transcripts in mouse preimplantation embryos and further analyze the abundance of circRNAs along development, the function of enriched genes, and sequence features of circRNAs. Our work is key to deciphering regulation mechanisms of circRNAs during mammalian early embryonic development. The online version of this article (doi:10.1186/s13059-015-0706-1) contains supplementary material, which is available to authorized users.