Efficient Transposition of a Single Minos Transposon Copy in the Genome of the Ascidian Ciona intestinalis With a Transgenic Line Expressing Transposase in Eggs
Efficient Transposition of a Single Minos Transposon Copy in the Genome of the Ascidian Ciona intestinalis With a Transgenic Line Expressing Transposase in Eggs
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DOI：
10.1002/dvdy.22254
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发表时间：
2010-04-01
影响因子：
2.5
通讯作者：
Sasakura, Yasunori
中科院分区：
文献类型：
--
作者：
Hozumi, Akiko;Kawai, Narudo;Sasakura, Yasunori
转座子转基因是研究遗传功能的一项重要技术。在海鞘中，利用Tc1/mariner转座子Minos进行种系转化的方法已经建立。为了完善这项转基因技术，需要一种系统来重新动员基因组中的单个Minos拷贝。在本研究中，我们用一个在鸡蛋中表达Minos转座酶的转基因株系建立了这样一个实验系统。在双转基因动物的卵子中，转座子被转座到Ciona基因组的新位置，从而产生了新的插入。一些新的插入导致了增强子检测。大多数新的插入位点定位在与转座子供体不同的染色体上。米诺斯的这一特征与睡美人转座子的这一特征形成了鲜明对比，后者会导致频繁的染色体内转座。发展动力学239：1076-1088,2010。(C)2010年Wiley-Liss公司
Transgenesis with transposons is an important technique for studying genetic functions. In the ascidian Ciona intestinalis, methods for germline transformation with the Tc1/mariner transposon Minos have been established. A system to remobilize a single Minos copy in the genome is needed to refine this transgenic technique. In this study, such an experimental system was established with a transgenic line expressing Minos transposase in eggs. In the eggs of a double transgenic animal from a cross between the egg transposase line and a transgenic line having a single Minos insertion, the transposon was transposed into new positions of the Ciona genome, thus creating new insertions. Some of the new insertions caused enhancer detection. The majority of the new insertion sites were mapped on different chromosomes from that of the transposon donor. This characteristic of Minos is in contrast to that of the Sleeping Beauty transposon, which causes frequent intrachromosomal transposition. Developmental Dynamics 239:1076-1088, 2010. (C) 2010 Wiley-Liss, Inc.