Analysis of the relationship between blue-light photon flux density and the photosynthetic properties of spinach (Spinacia oleracea L.) leaves with regard to the acclimation of photosynthesis to growth irradiance
Analysis of the relationship between blue-light photon flux density and the photosynthetic properties of spinach (Spinacia oleracea L.) leaves with regard to the acclimation of photosynthesis to growth irradiance
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DOI：
10.1111/j.1747-0765.2007.00150.x
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发表时间：
2007-08-01
影响因子：
2
通讯作者：
Kurata, Kenji
中科院分区：
文献类型：
--
作者：
Matsuda, Ryo;Ohashi-Kaneko, Keiko;Kurata, Kenji
蓝光被认为参与了光合作用对生长光照的适应。分析了蓝光强度对叶片光合特性的影响。菠菜（Spinacia oleracea L.）植物在蓝光和红光的混合光下生长，蓝光光子通量密度（PFD）为0、30、100和150 μ mol m（-2）s（-1），总光合PFD为300 μ mol m（-2）s（-1）。测定了白色光下的光饱和光合速率、单位叶面积的叶片氮含量、单位叶面积的叶片干重以及细胞色素f含量与捕光叶绿素结合蛋白11（LHCII）含量的比值。光合速率随着蓝光PFD的增加而增加，直到100 μ mol m（-2）s（-1），这与单位叶面积叶片氮含量的增加有关。然而，单位叶面积叶片氮含量的增加并不一定是由于单位叶面积叶片干重的增加。细胞色素f LHCII含量比线性增加，增加蓝光PFD高达100 μ mol M-2 S-1，表明植物生长在较高的蓝光PFD下，这个值类似于植物生长在较高的辐照度下的电子传输组件和捕光组件之间的N分配。这一结果表明，蓝光的水平只涉及在叶绿体水平的相对较低的生长辐照的驯化。
Blue light has been suggested to participate in the acclimation of photosynthesis to growth irradiance. We analyzed the effects of blue light intensity on the photosynthetic properties of leaves with regard to acclimation to irradiance. Spinach (Spinacia oleracea L.) plants were grown under mixtures of blue and red light with blue-light photon flux densities (PFDs) of 0, 30, 100 and 150 mu mol m(-2) s(-1) at a total photosynthetic PFD of 300 mu mol m(-2) s(-1). The light-saturated rate of photosynthesis under white light, leaf N content per unit leaf area, leaf dry weight per unit leaf area and the ratio of cytochrome (Cyt) f content to light-harvesting chlorophyll-binding protein of photosystem 11 (LHCII) content were evaluated. The photosynthetic rate tended to increase with increasing blue-light PFD up to 100 mu mol m(-2) s(-1), and this was associated with an increase in leaf N content per unit leaf area. However, the increase in leaf N content per unit leaf area did not necessarily result from an increase in leaf dry weight per unit leaf area. The Cyt f to LHCII content ratio increased linearly with increasing blue-light PFD up to 100 mu mol M-2 S-1, indicating that plants grown under higher blue-light PFD up to this value resembled plants grown under higher irradiance in terms of N partitioning between electron-transport components and light-harvesting components. This result suggests that the level of blue light is involved only in the acclimation to relatively low growth irradiances at the chloroplast level.