Structural Identification of the Oxygen-Evolving Apparatus on the Lumenal Surface of Freeze-Etched Thylakoid Membranes
Structural Identification of the Oxygen-Evolving Apparatus on the Lumenal Surface of Freeze-Etched Thylakoid Membranes
复制标题
冻蚀类囊体膜管腔表面放氧装置的结构鉴定
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
M. Seibert
中科院分区：
文献类型：
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作者：
L. Staehelin;M. Dewit;M. Seibert
在过去的10年里，光合作用的一般机制的理解取得了很大的进展，来自生物物理，生物化学和结构观察的整合。特别是，膜功能的生物物理测量的更准确的解释来自于通过生物化学和结构技术对类囊体膜的横向分化的证明。因此，已经发现光系统II（PSII）主要存在于（ca. 80-85%）位于类囊体膜的堆叠区域内，而光系统I（PSI）主要位于未堆叠的基质类囊体中。最近的冷冻断裂EM研究表明，EF颗粒是PSII复合物的结构等价物（综述见1）。由于这些EF颗粒是跨膜的性质和突出到内腔空间，我们一直有兴趣在相关的特征粒子聚集体[“四聚体”]上发现的冷冻蚀刻膜表面与特定的分子组成的PSII。
Over the past 10 years, much progress in the understanding of general mechanisms of photosynthesis has come from the integration of biophysical, biochemical, and structural observations. in particular, more accurate interpretations of biophysical measurements of membrane functions have come from the demonstration, by biochemical and structural techniques, of a lateral differentiation of thylakoid membranes. Thus, it has been found that photosystem II (PSII) resides primarily (ca. 80–85%) within the stacked regions of thylakoid membranes whereas photosystem I (PSI) has been located mostly in unstacked stroma thylakoids. More recent freeze-fracture EM studies have suggested that EF particles are structural equivalents of PSII complexes (reviewed in 1). Since these EF particles are transmembrane in nature and protrude into the lumenal space, we have been interested in correlating the characteristic particle aggregates [“tetramers”] found on freeze-etched membrane surfaces with specific molecular constituents of PSII.