Establishment of a patient-derived mucoepidermoid carcinoma cell line with the CRTC1-MAML2 fusion gene.
Establishment of a patient-derived mucoepidermoid carcinoma cell line with the CRTC1-MAML2 fusion gene.
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DOI：
10.3892/mco.2022.2508
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发表时间：
2022-03
影响因子：
1.2
通讯作者：
Nakano Y
中科院分区：
文献类型：
--
作者：
Noguchi K;Kanda S;Yoshida K;Funaoka Y;Yamanegi K;Yoshikawa K;Takaoka K;Kishimoto H;Nakano Y
粘液表皮样癌是最常见的大、小涎腺恶性肿瘤。手术切除是唯一的治愈性治疗方法，目前尚无有效的术后治疗方法。本研究报告了一个机构审查委员会批准的情况下，45岁的日本女性诊断为低度MEC在硬腭。行根治性切除、肩胛舌骨上颈淋巴结清扫和股前外侧皮瓣重建。然后从切除的肿瘤组织建立MEC细胞系。短串联重复序列分析证实了该细胞系的起源和真实性，该细胞系含有CRTC 1-MAML 2易位，这在MEC中经常观察到。双调蛋白（AREG），确定为CRTC 1-MAML 2融合基因的目标之一，在细胞系中表达。AREG受体，表皮生长因子受体（EGFR）也高度磷酸化。结果预测，肿瘤生长和存活所需的AREG-EGFR信号传导可能以细胞自主的方式在细胞系中被激活。由于AREG表达与EGFR靶向耐药性相关，该细胞系可能有助于确定MEC治疗的新策略。
Mucoepidermoid carcinoma (MEC) is the most common malignant tumor of the major and minor salivary glands. Surgical resection is the only curative treatment and there is no effective post-operative therapy for MEC. The present study reports an Institutional Review Board-approved case of a 45-year-old Japanese female diagnosed with low-grade MEC in the hard palate. Radical resection, supraomohyoid neck dissection and antero-lateral thigh flap reconstruction was performed. A MEC cell line was then established from the resected tumor tissue. Short tandem repeat profiling confirmed the origin and authenticity of the cell line, that harbors a CRTC1-MAML2 translocation, which is frequently observed in MEC. Amphiregulin (AREG), identified as one of the targets of the CRTC1-MAML2 fusion gene, was expressed in the cell line. The AREG receptor, epidermal growth factor receptor (EGFR) was also highly phosphorylated. The results predicted that AREG-EGFR signaling, which is required for tumor growth and survival, might be activated in the cell line in a cell-autonomous manner. As AREG expression is associated with EGFR-targeted drug resistance, this cell line might assist with the identification of novel strategies for MEC treatment.
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影响因子：
17.3
作者：
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通讯作者：
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影响因子：
2.2
作者：
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作者：
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