Dose and time effects of estrogen on expression of neuron-specific protein and cyclic AMP response element-binding protein and brain region volume in the medial amygdala of ovariectomized rats
Dose and time effects of estrogen on expression of neuron-specific protein and cyclic AMP response element-binding protein and brain region volume in the medial amygdala of ovariectomized rats
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DOI：
10.1159/000129498
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发表时间：
2008-01-01
影响因子：
4.1
通讯作者：
Cohen, Rochelle S.
中科院分区：
文献类型：
--
作者：
Fan, Lu;Hanbury, Rose;Cohen, Rochelle S.
尽管雌激素已被证明具有神经保护作用，但有关其对某些行为的影响的研究是矛盾的，报告既有改善作用，也有有害作用。影响激素功效的一个因素是雌激素疗法。我们报告了 10 μg 雌激素持续 14 天对大鼠内侧杏仁核 (MeA) 的环 AMP 反应元件结合蛋白 (CREB) 通路（包括脑源性神经营养因子）的影响。为了确定雌激素对 MeA 和杏仁核中央核 (CeA) 神经元数量和脑区体积的影响，我们使用体视学来测试各种雌激素方案对神经元特异性蛋白 (NeuN) 标记的神经元数量以及 MeA 和 CeA 脑区体积的影响。切除卵巢的大鼠注射媒介物14天，注射2.5μg苯甲酸雌二醇(E2)4或14天，或注射10μg雌激素14天。由于 NeuN 标记的神经元数量可能与神经元存活和 CREB 信号传导的上调有关，因此我们测试了这些方案对 MeA 和 CeA 中磷酸化 CREB (pCREB) 标记水平的影响。与载体或2.5μg雌激素持续4天相比，2.5μg雌激素持续14天的方案增加了MeA中NeuN标记的神经元和pCREB标记的细胞的平均数量。与媒介物或2.5μg雌激素4天相比，2.5μg雌激素14天的MeA体积增加。在 CeA 中未发现这些参数存在差异。这些数据表明雌激素对 MeA 与 CeA 中表达 NeuN 和 pCREB 的细胞的时间效应存在神经解剖学异质性。版权所有 (C) 2008 S. Karger AG，巴塞尔。
Although estrogen has been shown to be neuroprotective, studies concerning its effect on some behaviors are contradictory, reporting both ameliorative and detrimental effects. A factor involved in hormone efficacy is the estrogen regimen. We reported an effect of 10 mu g estrogen for 14 days on the cyclic AMP response element-binding protein (CREB) pathway, including brain-derived neurotrophic factor, in rat medial amygdala (MeA). To determine the effects of estrogen on neuronal numbers and brain region volume in MeA and central nucleus of the amygdala (CeA), we used stereology to test the effect of various estrogen regimens on the number of neuron-specific protein (NeuN)-labeled neurons and brain region volume of MeA and CeA. Ovariectomized rats were injected with vehicle for 14 days, 2.5 mu g estradiol benzoate (E2) for 4 or 14 days, or 10 mu g estrogen for 14 days. Because NeuN-labeled neuronal number may be related to neuronal survival and upregulation of CREB signaling, we tested the effect of these regimens on levels of phosphorylated CREB (pCREB) labeling in the MeA and CeA. The 2.5 mu g estrogen for 14 days regimen increased the mean number of NeuN-labeled neurons and pCREB-labeled cells in the MeA compared to vehicle or 2.5 mu g for 4 days. There was an increase in volume of the MeA with 2.5 mu g estrogen for 14 days compared to vehicle or 2.5 mu g for 4 days. No differences in these parameters were seen in CeA. These data indicate a neuroanatomical heterogeneity of a time effect of estrogen on cells expressing NeuN and pCREB in the MeA versus CeA. Copyright (C) 2008 S. Karger AG, Basel.