Extracellular matrix proteins and the dynamics of dentin formation
Extracellular matrix proteins and the dynamics of dentin formation
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DOI：
10.1080/03008200290000682
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发表时间：
2002-04-01
影响因子：
2.9
通讯作者：
McKee, MD
中科院分区：
文献类型：
--
作者：
Butler, WT;Brunn, JC;McKee, MD
牙本质发生涉及受控反应，当磷灰石晶体形成时，未矿化的前牙本质转化为牙本质。这个过程是动态的：成熟事件发生在前牙本质内，从近端层开始并进展到前牙本质-牙本质（PD）边界。一种类型的受控反应是通过裂解至少三个高度保守的肽键，将牙本质唾液酸磷蛋白（DSPP）蛋白水解加工成牙本质唾液酸蛋白（DSP）和牙本质磷蛋白（DPP）。我们假设该处理事件代表一个激活步骤，导致 DPP 的释放，DPP 对磷灰石晶体的形成和生长具有积极的影响。牙本质基质蛋白 1 (DPM1) 在骨中以加工片段（57-kD 蛋白质）的形式存在，在十二烷基硫酸钠聚丙烯酰胺凝胶电泳中在牙本质中观察到为 150-200 kD 的完整蛋白质。抗 57-kD 抗体可在骨、牙本质和细胞牙骨质中引发免疫反应。在骨骼中，反应性与骨细胞及其细胞过程有关。类似地，牙本质在成牙本质细胞、前牙本质和成牙本质细胞过程中显示出反应性。总之，将富含唾液酸的大蛋白加工成更小的片段可能是控制前牙本质向牙本质和类骨质向骨的转化的重要部分。
Dentinogenesis involves controlled reactions that result in conversion of unmineralized predentin to dentin when apatite crystals are formed. This process is dynamic: Maturation events occur within predentin beginning at the proximal layer and progressing to the predentin-dentin (PD) border. One type of controlled reaction is the proteolytic processing of dentin sialophosphoprotein (DSPP) to dentin sialoprotein (DSP) and dentin phosphoprotein (DPP), by cleavage of at least three highly conserved peptide bonds. We postulate that this processing event represents an activation step, resulting in release of DPP, which is active in its effects on formation and growth of apatite crystals. Dentin matrix protein 1 (DPM1), present as a processed fragment (57-kD protein) in bone, is seen in dentin on sodium dodecyl sulfate polyacrylamide gel electrophoresis as one intact protein of 150-200 kD. Anti-57-kD antibodies elicit immunoreactivity in bone, dentin, and cellular cementum. In bone, the reactivity is associated with osteocytes and their cell processes. Similarly, dentin shows reactivity in odontoblasts, predentin, and the odontoblast processes. In summary, the processing of large sialic acid-rich proteins into smaller fragments may be an important part of the controlled conversion of predentin to dentin and osteoid to bone.