Isolation and characterization of a Tritordeum cDNA encoding S-adenosylmethionine decarboxylase that is circadian-clock-regulated
Isolation and characterization of a Tritordeum cDNA encoding S-adenosylmethionine decarboxylase that is circadian-clock-regulated
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DOI：
10.1007/bf00020812
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发表时间：
1996-03
影响因子：
5.1
通讯作者：
T. Dresselhaus;P. Barceló;C. Hagel;H. Lörz;K. Humbeck
中科院分区：
文献类型：
--
作者：
T. Dresselhaus;P. Barceló;C. Hagel;H. Lörz;K. Humbeck
对从二倍体野生大麦和四倍体小麦品系的六倍体杂交种Tritordeum获得的外植体文库进行差异筛选获得的两个克隆47/11和50A的序列分析表明，这两个克隆包含相同的开放阅读框架(ORF)。该ORF序列与Dicot-Adenosyl蛋氨酸脱羧酶(SAMDC)基因序列高度相似，并含有在所有已知SAMDC序列中高度保守的区域。进一步证明，克隆47/11和50A所代表的序列来源于野生大麦(Hordeum Chilense)基因组，其中以单拷贝基因形式存在。Northern分析表明，相应的转录本对伤害作出反应而积累，转录本水平随昼夜节律变化，在光照期间中期达到峰值。这种周期性在恒定的光中持续，但在恒定的黑暗中改变。
Sequence analysis of the two cDNA clones 47/11 and 50A which were isolated by differential screening of an explant cDNA library obtained from the monocot Tritordeum (hexaploid hybrid of diploid wild barley and tetraploid wheat lines) reveals that both clones include the same open reading frame (ORF). The sequence of this ORF shows a high degree of similarity with dicotS-adenosylmethionine decarboxylase (SAMDC) gene sequences and contains regions highly conserved in all known SAMDC sequences. It is further shown that the sequence represented by the cDNA clones 47/11 and 50A is derived from the wild barley (Hordeum chilense) genome, where it is present as a single-copy gene. Northern analyses indicate the corresponding transcript to accumulate in response to wounding and the transcript level changes with a circadian rhythm, having a peak in the middle of the light period. The periodicity continues in constant light, but is changed in constant darkness.