OBSERVATIONS ON THE OXIDATION OF HOMOGENTISIC ACID IN URINE
OBSERVATIONS ON THE OXIDATION OF HOMOGENTISIC ACID IN URINE
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DOI：
10.1042/bj0500274
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发表时间：
1951-01-01
影响因子：
4.1
通讯作者：
STANIER, WM
中科院分区：
文献类型：
--
作者：
CONSDEN, R;FORBES, HAW;STANIER, WM
描述了黑黑豆酸和苯醌乙酸的纸色谱法，并给出了检测方法。纸色谱分离后尿液中尿黑酸的含量。 u.-v。检测了这两种物质的吸收光谱。通过 u.-v 方法跟踪氧化条件下尿液中尿黑酸的行为。分光光度法。如果存在亚硝酸或当尿液呈碱性时，尿液中就会转化为醌。通过纸层析和分光光度法，发现风湿热病例、坏血病豚鼠和实验大鼠的尿液中不存在尿黑酸和苯醌乙酸。高铁血球蛋白血症。
Paper chromatography of homogentisic acid and benzo-quinoneacetic acid is described, and a method is given for the detn. of homogentisic acid in urine after separation on paper chromatograms. The u.-v. absorption spectra of these 2 substances were detd. The behavior of homogentisic acid in urines under oxidizing conditions was followed by means of u.-v. spectrophotometry. Conversion to the quinone takes place in urine, if nitrous acid is present or when the urine is made alkaline. By paper chromatography and by spectrophotometry, homogentisic acid and benzoquinoneacetic acid were found to be absent from the urines of rheumatic fever cases, scorbutic guinea pigs and rats with exptl. methemo-globinaemia.